Anti-Human BMP-9

Product No.: B648


Clone 360107 Target BMP-9 Formats AvailableView All Product Type Monoclonal Antibody Alternate Names Bone Morphogenetic Protein-9, Growth/Differentiation Factor-2, GDF2, BMP9 Isotype IgG2b Applications ELISA Cap , N , WB


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Data


Antibody Details Product Details Reactive Species Human Host Species Mouse Immunogen Purified Recombinant Human BMP-9 (Accession # Q9UK05) Endotoxin Level <0.1 EU/µg as determined by the LAL method Formulation This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.4 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present. Storage and Handling The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2828305 Applications and Recommended Usage? Quality Tested by Leinco ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 2-8 µg/ml. A suitable detection antibody is PN:B647. Western Blotting: To detect Human BMP-9 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Human BMP-9 is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:B624. Additional Applications Reported In Literature ? Neutralization: This antibody is useful for neutralization of Human BMP-9 bioactivity. The antibody dose required to neutralize 50% (ND₅₀) of the biological activity of Human BMP-9 (at 5 ng/ml) is 0.01-0.04 µg/ml. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. Description Description Specificity Mouse Anti-Human Bone Morphgenetic Protein 9 (BMP-9) (Clone 360107) recognizes an epitope on Human BMP-9. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. Background Bone morphogenetic protein (BMP-9) is also known as growth differentiation factor 2 (GDF2) is a protein that in humans encoded by the GDF2 gene. GDF2 belongs to the transforming growth factor beta (TGF-β) superfamily. BMP9 has a role in inducing and maintaining the ability of embryonal and adult tissues. Unlike with other BMP family proteins, the propeptide does not interfere with the biological activity of BMP9 and remains associated with the mature peptide after proteolytic cleavage. Another suggested role of BMP-9 could be a potent inducer of hepcidin in liver cells and could regulate iron metabolism. Mature human and mouse BMP-9 share 96% amino acid sequence identity. PubMed BMP-9 NCBI Gene Bank ID 2658 UniProt.org Information on Uniprot.org References & Citations View product citations for antibody B648 on CiteAb Technical Protocols N Certificate of Analysis Request COA

Antibody Details

Product Details

Reactive Species

Human

Host Species

Mouse

Immunogen

Purified Recombinant Human BMP-9 (Accession # Q9UK05)

Endotoxin Level

<0.1 EU/µg as determined by the LAL method

Formulation

This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.4 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.

Storage and Handling

The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.

Country of Origin

USA

Shipping

Next Day Ambient

RRIDAB_2828305

Applications and Recommended Usage?
Quality Tested by Leinco

ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 2-8 µg/ml. A suitable detection antibody is PN:B647.
Western Blotting: To detect Human BMP-9 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Human BMP-9 is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:B624.

Additional Applications Reported In Literature ?

Neutralization: This antibody is useful for neutralization of Human BMP-9 bioactivity. The antibody dose required to neutralize 50% (ND₅₀) of the biological activity of Human BMP-9 (at 5 ng/ml) is 0.01-0.04 µg/ml.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Mouse Anti-Human Bone Morphgenetic Protein 9 (BMP-9) (Clone 360107) recognizes an epitope on Human BMP-9. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Background

Bone morphogenetic protein (BMP-9) is also known as growth differentiation factor 2 (GDF2) is a protein that in humans encoded by the GDF2 gene. GDF2 belongs to the transforming growth factor beta (TGF-β) superfamily. BMP9 has a role in inducing and maintaining the ability of embryonal and adult tissues. Unlike with other BMP family proteins, the propeptide does not interfere with the biological activity of BMP9 and remains associated with the mature peptide after proteolytic cleavage. Another suggested role of BMP-9 could be a potent inducer of hepcidin in liver cells and could regulate iron metabolism. Mature human and mouse BMP-9 share 96% amino acid sequence identity.

PubMed

BMP-9

NCBI Gene Bank ID

2658

UniProt.org

Information on Uniprot.org