Pricing & Details


Product No.C385 Protein CT-1 Formats AvailableView All Product Type Polyclonal Antibody Alternate Names Cardiotrophin-1, CTF1 Applications N , WB Submit A Review	Printable Datasheet Printable SDS

Prod No.	Size	Price	Avail.	Qty	Add to cart
C385-25 µg	25 µg	$190.00	In stock	Max: Min: 1 Step: 1	Add to Cart
C385-100 µg	100 µg	$455.00	In stock	Max: Min: 1 Step: 1	Add to Cart

Antibody Details

Product Details

Reactivity Species

Human

Host Species

Goat

Immunogen

E. coli-Derived Recombinant Human Cardiotrophin-1

Endotoxin Level

<0.1 EU/µg as determined by the LAL method

Formulation

This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.

Storage and Handling

The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.

Country of Origin

USA

Shipping

Next Day Ambient

RRIDAB_2829688

Applications and Recommended Usage?
Quality Tested by Leinco

Western Blotting: To detect Human CT-1 this polyclonal antibody can be used at a concentration of 0.1 - 0.2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as PN:G505 and a chromogenic substrate such as PN:T343. The detection limit for Human CT-1 is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.

Other Applications Reported In Literature ?

Neutralization: This antibody is useful for neutralization of Human CT-1 bioactivity. The antibody dose required to neutralize 50% (ND₅₀) of the biological activity of Human CT-1 (at 15 ng/ml) is 0.1 - 0.3 µg/ml.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Rabbit Anti-Human Cardiotrophin-1 (CT-1) recognizes Human CT-1. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.

Antigen Distribution

Human CT-1 is expressed in various tissues including the adult heart, skeletal muscle, ovary, colon, prostate and fetal lungs.

Background

Human CT-1 is a member of the IL-6 family of cytokines which also includes LIF, CNTF, OSM (Oncostatin M), IL-11, IL-6 and possibly NT-1/ BSF-3. CT-1 is a pleiotropic cytokine which signals through the LIF receptor and the gp130 receptor subunit. CT-1 has the ability to induce cardiac myocyte hypertrophy, and enhances the survival of cardiomyocyte and different neuronal populations. Biologically active human CT-1 is synthesized as a 201 amino acid polypeptide lacking a hydrophobic N-terminal secretion signal sequence.

Human and mouse CT-1 share 80% aa sequence identity and exhibit cross-species activity.