Anti-Human Caspase-12 (Intermediate Domain)

PN:C1132

This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.

This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.

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Rabbit Anti-Human Caspase-12 recognizes an epitope in the intermediate domain of mouse, rat and human Caspase-12. This polyclonal antibody was purified using affinity chromatography.

Three distinct signaling pathways lead to programmed cell death (apoptosis). The death receptor and mitochondrion pathways are the mains, in which the key apoptotic proteases capase-8 and caspase-9, respectively, are involved. The endoplasmic reticulum (ER) stress is the third apoptotic pathway and caspase-12 is involved.^1,2 Caspase-12 is localized to the ER but not to cytoplasm or mitochondrion. Caspase-12 is activated by ER stress, including disruption of ER calcium homeostasis, and mediates ER stress-induced apoptosis. Caspase-12 is co-localized to the ER with several proteins that are involved in Alzheimer's disease including secretase presenilin and β-amyloid precursor protein (APP). Caspase-12 mediates cytotoxicity induced by amyloid-β. Caspase-12 is ubiquitously expressed in mouse tissues.¹

1. Nakagawa, T. et al. (2000) Nature 403:98-103.
2. Mehmet, H. (2000) Nature 403:29-30
3. Van de Craen, M. et al. (1997) FEBS Lett 403:61-9