Anti-Human CCL14 (Clone 256409)

Product No.: C1604


Clone 256409 Target CCL14 Formats AvailableView All Product Type Monoclonal Antibody Alternate Names Chemokine (C-C Motif) Ligand 14, HCC-1, HCC-3 Isotype IgG2a Applications ELISA Cap , N , WB


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Data


Antibody Details Product Details Reactive Species Human Host Species Mouse Immunogen Purified Recombinant Human CCL14 (Accession # NP_116738) Endotoxin Level <0.1 EU/µg as determined by the LAL method Formulation This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present. Storage and Handling The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2828667 Applications and Recommended Usage? Quality Tested by Leinco ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 2-8 µg/ml. A suitable detection antibody is PN:H256 at a concentration of approximately 0.1-0.4 µg/ml. A suggested standard for this assay is PN:H257. Western Blotting: To detect Human CCL14 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Human CCL14 is 1 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:H257. Additional Applications Reported In Literature ? Neutralization: This antibody is useful for neutralization of Human CCL14 bioactivity. The antibody dose required to neutralize 50% (ND₅₀) of the biological activity of Human CCL14 (at 10 ng/ml) is 0.5-2.5 µg/ml. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. Description Specificity Mouse Anti-Human CCL14 (Clone 256409) recognizes an epitope on Human CCL14. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. PubMed CCL14 NCBI Gene Bank ID 6358 UniProt.org Information on Uniprot.org Research Area Chemokine References & Citations View product citations for antibody PRODUCT_CODE on CiteAb Technical Protocols N Certificate of Analysis Request COA

Antibody Details

Product Details

Reactive Species

Human

Host Species

Mouse

Immunogen

Purified Recombinant Human CCL14 (Accession # NP_116738)

Endotoxin Level

<0.1 EU/µg as determined by the LAL method

Formulation

This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.

Storage and Handling

The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.

Country of Origin

USA

Shipping

Next Day Ambient

RRIDAB_2828667

Applications and Recommended Usage?
Quality Tested by Leinco

ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 2-8 µg/ml. A suitable detection antibody is PN:H256 at a concentration of approximately 0.1-0.4 µg/ml. A suggested standard for this assay is PN:H257.
Western Blotting: To detect Human CCL14 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Human CCL14 is 1 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:H257.

Additional Applications Reported In Literature ?

Neutralization: This antibody is useful for neutralization of Human CCL14 bioactivity. The antibody dose required to neutralize 50% (ND₅₀) of the biological activity of Human CCL14 (at 10 ng/ml) is 0.5-2.5 µg/ml.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Mouse Anti-Human CCL14 (Clone 256409) recognizes an epitope on Human CCL14. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

PubMed

CCL14

NCBI Gene Bank ID

6358

UniProt.org

Information on Uniprot.org

Research Area

Chemokine