Pricing & Details

Product No.C1403
Clone
52130
Protein
CCL15
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
MIP-5, SCYA15, HCC-2, NCC-3, SCYL3, Lkn-1, MIP-1d, HMRP-2B, CCL15
Isotype
Mouse
IgG1
Applications
ELISA Cap
,
N
,
WB
Prod No.
Size
Price
Avail.
Qty
Add to cart
C1403-25 µg
25 µg
$185.00
In stock
Max:
Min: 1
Step: 1
C1403-500 µg
500 µg
$515.00
In stock
Max:
Min: 1
Step: 1
Bulk quantities available. Contact us for pricing.

Antibody Details

Product Details

Reactivity Species
Human
Host Species
Mouse
Immunogen
Purified Recombinant Human MIP-5 (Accession # Q16663.2)
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Formulation
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.4 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Storage and Handling
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 2-8 µg/ml. A suitable detection antibody is PN:M200 at a concentration of approximately 0.1-0.4 µg/ml. A suggested standard for this assay is PN:M1239.
Western Blotting: To detect Human MIP-5 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Human MIP-5 is 50 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:M1239.
Other Applications Reported In Literature ?
Neutralization: This antibody is useful for neutralization of Human CCL15 bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Human CCL15 (at 16 ng/ml) is 3-15 µg/ml.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Mouse Anti-Human CCL15 (Clone 52130), also know as MIP-1 delta recognizes an epitope on Human CCL15. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Background
CCL15 is a CC chemokine is expressed at high levels in liver, intestine, and in lung leukocytes. It induces chemotaxis of monocytes, T-lymphocytes and eosinophils. MIP-5 competes with MIP-1-alpha for binding to CC-Chemokine receptor CCR1, and with MCP-3 for binding to CCR3.1

Antigen Details

Protein
PubMed

References & Citations

1. Coulin, F. et al. (1997) J. Biochem. 248: 507
Elisa Sandwich Protocol
General Western Blot Protocol
Prod No.
Description
L249

Formats Available

Prod No.
Description
C1403
Products are for research use only. Not for use in diagnostic or therapeutic procedures.