Purified Recombinant Human CCL17 (Accession # Q92583.1)
<0.1 EU/µg as determined by the LAL method
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.4 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Storage and Handling
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Country of Origin
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
Other Applications Reported In Literature ?
Neutralization: This antibody is useful for neutralization of Human CCL17 bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Human CCL17 (at 10.0 ng/ml) is 0.4 - 2.0 µg/ml.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Mouse Anti-Human Chemokine (C-C Motif) Ligand 17 (CCL17) (Clone 54026) recognizes an epitope on Human CCL17. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Chemokine (C-C motif) ligand 17 (CCL17) is a small cytokine belonging to the CC chemokine and is expressed constitutively in thymus, but only transiently in phytohemagglutinin-stimulated peripheral blood mononuclear cells.1 This chemokine specifically binds and induces chemotaxis in T cells and elicits its effects by interacting with the chemokine receptor CCR4.1,2 CCL17 may be a useful serological marker and may facilitate an assessment of the degree of disease activity in systemic lupus erythematosus (SLE).3 It may also participate in the pathogenesis of skin diseases such as atopic dermatitis (AD) by regulating both allergic and irritant inflammation.4
References & Citations
1. Yoshie, O. et al. (1996) J Biol Chem. 271: 21514
2. Yoshie, O. et al. (1997) J Biol Chem. 272: 15036
3. Kamatani, N. et al. (2003) J Rheumatology 30: 2369
4. Kunihiko, K. et al. (2006) Eur J Immunol. 36: 2116
Products are for research use only. Not for use in diagnostic or therapeutic procedures.