Anti-Human CCL17 (Clone 54026)

Purified Recombinant Human CCL17 (Accession # Q92583.1)

This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.4 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.

The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.

Next Day Ambient

Neutralization: This antibody is useful for neutralization of Human CCL17 bioactivity. The antibody dose required to neutralize 50% (ND₅₀) of the biological activity of Human CCL17 (at 10.0 ng/ml) is 0.4 - 2.0 µg/ml.

Mouse Anti-Human Chemokine (C-C Motif) Ligand 17 (CCL17) (Clone 54026) recognizes an epitope on Human CCL17. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Chemokine (C-C motif) ligand 17 (CCL17) is a small cytokine belonging to the CC chemokine and is expressed constitutively in thymus, but only transiently in phytohemagglutinin-stimulated peripheral blood mononuclear cells.¹ This chemokine specifically binds and induces chemotaxis in T cells and elicits its effects by interacting with the chemokine receptor CCR4.^1,2 CCL17 may be a useful serological marker and may facilitate an assessment of the degree of disease activity in systemic lupus erythematosus (SLE).³ It may also participate in the pathogenesis of skin diseases such as atopic dermatitis (AD) by regulating both allergic and irritant inflammation.⁴

1. Yoshie, O. et al. (1996) J Biol Chem. 271: 21514
2. Yoshie, O. et al. (1997) J Biol Chem. 272: 15036
3. Kamatani, N. et al. (2003) J Rheumatology 30: 2369
4. Kunihiko, K. et al. (2006) Eur J Immunol. 36: 2116