Anti-Human CCL24-Biotin

Anti-Human CCL24-Biotin

Product No.: E125

[product_table name="All Top" skus="E125"]

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Target
CCL24
Product Type
Polyclonal Antibody
Alternate Names
Eotaxin-2, MPIF-2, CkB-6, SCYA24
Applications
ELISA Det
,
WB

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Antibody Details

Product Details

Reactive Species
Human
Host Species
Goat
Immunogen
E. coli-Derived Recombinant Human CCL24
Formulation
This biotinylated antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 50 µg of bovine serum albumin per µg of antibody with no calcium, magnesium, or preservatives present.
State of Matter
Lyophilized
Storage and Handling
The lyophilized, biotinylated antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for up to twelve months from date of receipt. The reconstituted bioin conjugate can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted conjugate, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
Western Blotting: To detect Human Eotaxin-2 this biotin conjugate can be used at a concentration of 0.1 - 0.2 µg/ml. This biotin conjugate should be used in conjunction with compatible second-step reagents such as PN:A106 and a chromogenic substrate such as PN:T343. The detection limit for Human Eotaxin-2 is 2 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
ELISA Detection: Suitable for use at concentration of 0.1-0.4 µg/mL. Use in combination with: Capture Reagent: Human CCL24/Eotaxin‑2/MPIF‑2 Antibody. Standard: Recombinant Human CCL24/Eotaxin-2/MPIF-2 Protein
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Goat Anti-Human Eotaxin-2 recognizes Human Eotaxin-2. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Background
Eotaxin-2 is a CC chemokine that is a specific agonist for the chemokine receptor CCR3. It is produced by activated monocytes and T lymphocytes. Eotaxin-2 induces chemotaxis of eosinophils as well as basophils and the release of histamine and leukotriene C4 from basophils. Eotaxin-2 has the same efficacy as eotaxin, but is somewhat less potent. 1
Additionally, Eotaxin-2 inhibits the proliferation of multipotential hematopoietic progenitor cells.

Antigen Details

PubMed
NCBI Gene Bank ID

References & Citations

1. Baggiolini, M. et al. (1997) J. Exp. Med. 185: 2171
ELISA Det
General Western Blot Protocol

Certificate of Analysis

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Prod No.
Description
E125
Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.