Anti-Human CCL28 (Clone 62705)

Pricing & Details

Product No.C1451
Clone
62705
Protein
CCL28
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
Chemokine (C-C Motif) Ligand 28, SCYA28, MEC, CCK1
Isotype
Mouse
IgG1
Applications
N
,
WB
Prod No.
Size
Price
Avail.
Qty
Add to cart
C1451-25 µg
25 µg
$205.00
In stock
Max:
Min: 1
Step: 1
C1451-500 µg
500 µg
$513.00
In stock
Max:
Min: 1
Step: 1
Bulk quantities available. Contact us for pricing.

Antibody Details

Product Details

Reactivity Species
Human
Host Species
Mouse
Immunogen
E. coli - Derived Recombinant Human CCL28 (Accession # Q9NRJ3)
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Formulation
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.4 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Storage and Handling
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
Western Blotting: To detect Human CCL28 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for rhCCL28 is approximately 20 ng/lane and 50 ng/lane under non-reducing and reducing conditions, respectively. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
Other Applications Reported In Literature ?
Neutralization: This antibody is useful for neutralization of Human CCL28 bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Human CCL28 (at 4 ng/ml) is 15 - 45 µg/ml.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Mouse Anti-Human CCL28 (Clone 62705) recognizes an epitope on Human CCL28. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Background
CCL28, also known as mucosae-associated epithelial chemokine (MEC), CCK1 and SCYA28, is a chemokine. CCL28 regulates the chemotaxis of cells that express the chemokine receptors CCR3 and CCR10. CCL28 is expressed by columnar epithelial cells in the gut, lung, breast and the salivary glands and drives the mucosal homing of T and B lymphocytes that express CCR10, and the migration of eosinophils expressing CCR3.1,2,3 CCL28 is constitutively expressed in the colon, but its levels can be increased by pro-inflammatory cytokines and certain bacterial products implying a role in effector cell recruitment to sites of epithelial injury.3 CCL28 is a key regulator of IgA antibody-secreting cells (ASCs) accumulation in the mammary gland and thus controls the passive transfer of IgA antibodies from mother to infant.4

Antigen Details

Protein
PubMed
NCBI Gene Bank ID

References & Citations

1. Kunkel, SL. et al. (2003) J Clin. Invest. 111: 1001
2. John, AE. et al. (2005) J. Pathol. 166(2): 345
3. Hieshima, GB. et al. (2003) J Immunol. 170: 1452
4. Butcher, EC. et al. (2004) J Exp. Med. 200: 805
General Western Blot Protocol

Formats Available

Prod No.
Description
C1451
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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