Anti-Mouse CCL28

Anti-Mouse CCL28

Product No.: C1466

[product_table name="All Top" skus="C1466"]

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Target
CCL28
Product Type
Polyclonal Antibody
Alternate Names
Chemokine (C-C Motif) Ligand 28, SCYA28, MEC, CCK1
Applications
N
,
WB

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Antibody Details

Product Details

Reactive Species
Mouse
Host Species
Mouse
Immunogen
E. coli - Derived Recombinant Mouse CCL28
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Formulation
This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.
State of Matter
Lyophilized
Storage and Handling
The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
Western Blotting: To detect Mouse CCL28 this polyclonal antibody can be used at a concentration of 0.1 - 0.2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Mouse CCL28 is 2 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
Additional Applications Reported In Literature ?
Neutralization: This antibody is useful for neutralization of Mouse CCL28 bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Mouse CCL28 (at 2 ng/ml) is 5 - 25 µg/ml.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Description

Specificity
Mouse Anti-Mouse CCL28 recognizes Mouse CCL28. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Background
CCL28, also known as mucosae-associated epithelial chemokine (MEC), CCK1 and SCYA28, is a chemokine. CCL28 regulates the chemotaxis of cells that express the chemokine receptors CCR3 and CCR10. CCL28 is expressed by columnar epithelial cells in the gut, lung, breast and the salivary glands and drives the mucosal homing of T and B lymphocytes that express CCR10, and the migration of eosinophils expressing CCR3.1,2,3 CCL28 is constitutively expressed in the colon, but its levels can be increased by pro-inflammatory cytokines and certain bacterial products implying a role in effector cell recruitment to sites of epithelial injury.3 CCL28 is a key regulator of IgA antibody-secreting cells (ASCs) accumulation in the mammary gland and thus controls the passive transfer of IgA antibodies from mother to infant.4
PubMed
NCBI Gene Bank ID

References & Citations

1. Kunkel, SL. et al. (2003) J Clin. Invest. 111: 1001
2. John, AE. et al. (2005) J. Pathol. 166(2): 345
3. Hieshima, GB. et al. (2003) J Immunol. 170: 1452
4. Butcher, EC. et al. (2004) J Exp. Med. 200: 805
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General Western Blot Protocol

Certificate of Analysis

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Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.