Anti-Human CCL28

Pricing & Details

Product No.C1474
Protein
CCL28
Formats AvailableView All
Product Type
Polyclonal Antibody
Alternate Names
Chemokine (C-C Motif) Ligand 28, SCYA28, MEC, CCK1
Applications
N
,
WB
Prod No.
Size
Price
Avail.
Qty
Add to cart
C1474-25 µg
25 µg
$185.00
In stock
Max:
Min: 1
Step: 1
C1474-100 µg
100 µg
$421.00
In stock
Max:
Min: 1
Step: 1
Bulk quantities available. Contact us for pricing.

Antibody Details

Product Details

Reactivity Species
Human
Host Species
Goat
Immunogen
E. coli-Derived Recombinant Human CCL28
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Formulation
This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.4 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.
Storage and Handling
The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
Western Blot: Suitable for use at concentration of 0.1 µg/mL.
Other Applications Reported In Literature ?
Neutralization: Measured by its ability to neutralize CCL28-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with mouse CCR10. The Neutralization Dose (ND50) is typically 40-100 µg/mL in the presence of 4 µg/mL Recombinant Human CCL28.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Goat Anti-Human CCL28 recognizes Human CCL28. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Background
CCL28, also known as mucosae-associated epithelial chemokine (MEC), CCK1 and SCYA28, is a chemokine. CCL28 regulates the chemotaxis of cells that express the chemokine receptors CCR3 and CCR10. CCL28 is expressed by columnar epithelial cells in the gut, lung, breast and the salivary glands and drives the mucosal homing of T and B lymphocytes that express CCR10, and the migration of eosinophils expressing CCR3.1,2,3 CCL28 is constitutively expressed in the colon, but its levels can be increased by pro-inflammatory cytokines and certain bacterial products implying a role in effector cell recruitment to sites of epithelial injury.3 CCL28 is a key regulator of IgA antibody-secreting cells (ASCs) accumulation in the mammary gland and thus controls the passive transfer of IgA antibodies from mother to infant.4

Antigen Details

Protein
PubMed
NCBI Gene Bank ID

References & Citations

1. Kunkel, SL. et al. (2003) J Clin. Invest. 111: 1001
2. John, AE. et al. (2005) J. Pathol. 166(2): 345
3. Hieshima, GB. et al. (2003) J Immunol. 170: 1452
4. Butcher, EC. et al. (2004) J Exp. Med. 200: 805
General Western Blot Protocol
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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