Anti-Human CD166 (ALCAM) – Dylight® 550

Anti-Human CD166 (ALCAM) – Dylight® 550

Product No.: C721

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Clone
3A6
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
CD6 ligand, Activated Leukocyte Cell Adhesion Molecule (ALCAM)
Isotype
IgG1 κ
Applications
FC
,
IF
,
IF Microscopy
,
IHC
,
IP

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Select Product Size
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Antibody Details

Product Details

Reactive Species
Human
Host Species
Mouse
Immunogen
Cultured human thymic epithelial cells
Product Concentration
0.2 mg/ml
Formulation
This DyLight® 550 conjugate is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Handling
This DyLight® 550 conjugate is stable when stored at 2-8°C. Do not freeze.
Country of Origin
USA
Excitation Laser
Yellow Laser (563 nm)
Applications and Recommended Usage?
Quality Tested by Leinco
FC
Additional Applications Reported In Literature ?
IHC,
IP,
IF,
IF Microscopy
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
3A6 activity is directed against human CD166 (ALCAM) and cross-reacts with ovine tissues.
Background
Activated leukocyte cell adhesion molecule (ALCAM) is a member of the immunoglobulin superfamily and a cell surface glycoprotein1. In normal physiology, ALCAM functions in cell adhesion, is known to promote T cell activation and proliferation by interacting with CD6, and functions in angiogenesis, monocyte transmigration, leukocyte intravasation across the blood-brain barrier, hematopoiesis, neurite extension, osteogenesis, and embryonic implantation in the uterus. In cancer, ALCAM is a prognostic marker of disease progression and acts as a modulator of progression by controlling cell proliferation, adhesion, migration, and invasion.

ALCAM participates in homophilic ALCAM-ALCAM interactions as well as numerous heterophilic interactions1. Ligands include CD6, galectin-8, endophilin-A3/galectin-8, CD9, S100B, and ezrin. Additionally, SOSTDC1 is a novel ligand of ALCAM that promotes invasion and facilitates liver metastasis in colorectal cancer through activation of the Src-P13K/AKT pathways2.

ALCAM is a type I transmembrane molecule with a large glycosylated extracellular domain1. Two isoforms have been confirmed at the protein level: ALCAM-Iso1, which is the full length isoform, and ALCAM-Iso2, which lacks exon 13. ALCAM is proteolytically cleaved at its extracellular domain by the transmembrane metalloprotease ADAM17, with ALCAM-Iso2 more susceptible to cleavage.

3A6 was produced by immunizing mice with human thymic epithelial cells and then fusing spleen cells with P3X63Ag8 myeloma cells3. 3A6 cross reacts with ovine mesenchymal stromal cells from iliac crest bone marrow aspirates4.
Antigen Distribution
CD166 is expressed on neurons, activated leukocytes, hematopoietic stem cells, mesenchymal stem cells, bone marrow stromal cells, activated T cells, activated B cells, activated monocytes, thymic epithelial cells, vascular endothelial cells, fibroblasts, keratinocytes, myeloid progenitors, tumor cells, and cancer stem cells.

Antigen Details

NCBI Gene Bank ID

References & Citations

1. Ferragut F, Vachetta VS, Troncoso MF, et al. Cytokine Growth Factor Rev. 61:27-37. 2021.
2. Bartolomé RA, Pintado-Berninches L, Jaén M, et al. Oncogene. 39(38):6085-6098. 2020.
3. Patel DD, Fong AM, Mann KP, et al. CD166 Workshop: Tissue distribution and functional analysis of antibodies reactive for CD166, a ligand for CD6. In: Kishimoto T, editor. Leukocyte Typing IV. Oxford: Oxford University Press; 1997. P.461-464.
4. Sanjurjo-Rodríguez C, Castro-Viñuelas R, Hermida-Gómez T, et al. PLoS One. 12(1):e0171231. 2017.
5. Piazza T, Cha E, Bongarzone I, et al. J Cell Sci. 118(Pt 7):1515-1525. 2005.
6. Tondreau T, Dejeneffe M, Meuleman N, et al. BMC Genomics. 9:166. 2008.
7. Srouji S, Kizhner T, Ben David D, et al. Calcif Tissue Int. 84(2):138-145. 2009.
8. Katsube Y, Kotobuki N, Tadokoro M, et al. Gene Ther. 17(4):494-502. 2010.
9. Brune JC, Tormin A, Johansson MC, et al. Int J Cancer. 129(2):319-330. 2011.
10. Ali H, Al-Yatama MK, Abu-Farha M, et al. PLoS One. 10(4):e0122465. 2015.
11. Prins HJ, Schulten EA, Ten Bruggenkate CM, et al. Stem Cells Transl Med. 5(10):1362-1374. 2016.
12. Fridriksdottir AJ, Kim J, Villadsen R, et al. Nat Commun. 6:8786. 2015.
13. Gong B, Zheng L, Lu Z, et al. Mol Med Rep. 23(1):43. 2021.
14. Yeh SP, Chang JG, Lin CL, et al. Leukemia. 19(8):1505-1507. 2005.
15. Levesque MC, Heinly CS, Whichard LP, et al. Arthritis Rheum. 41(12):2221-2229. 1998.
16. Ishiguro F, Murakami H, Mizuno T, et al. J Thorac Oncol. 7(5):890-899. 2012.
17. Bhattacharya S, Mathew G, Ruban E, et al. J Proteome Res. 9(12):6112-6125. 2010.
Flow Cytometry
IF
IF Microscopy
IHC
Immunoprecipitation Protocol

Certificate of Analysis

Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.