Anti-Human CD22 – Purified

Anti-Human CD22 – Purified

Product No.: C179

[product_table name="All Top" skus="C179"]

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Clone
RFB-4
Target
CD22
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
BL-CAM, MGC130020, SIGLEC2
Isotype
IgG1
Applications
FC
,
IHC FF

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Antibody Details

Product Details

Reactive Species
Human
Host Species
Mouse
Product Concentration
0.5 mg/ml
Formulation
This purified antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Handling
This purified antibody is stable when stored at 2-8°C. Do not freeze.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
FC The suggested concentration for this RFB-4 antibody for staining cells in flow cytometry is ≤ 1.0 μg per 106 cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application.
Additional Applications Reported In Literature ?
IHC (Frozen)
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Mouse Anti-Human CD22 (Clone RFB-4) recognizes Human CD22. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. Specificity: Anti-Human CD22 recognizes the (Mr 135 kDa) cytoplasmic antigen of all B-lymphocytes. The CD22 antigen is only expressed on the cell surface of mature B-lymphocytes.
Antigen Distribution
The CD22 antigen is expressed on >90% of peripheral blood B-cells.2 This antigen is not present on T-cells, monocytes or macrophages. CD22 is expressed on most peripheral B-cell lymphomas and leukemias including hairy cell leukemia, but not on the immature bone marrow type of leukemia.
Background
Cluster of differentiation 22 also known as CD22 is a B-cell-restricted transmembrane protein, which acts as a negative regulator of B-cell signalling. CD22 also has lectin-like adhesive properties.2 CD22 is a good target to shorten the time required for Ab production, thereby augmenting host defense against acute infectious diseases as "universal vaccination."3

Antigen Details

Ligand/Receptor
CD45RO, CD75
PubMed
NCBI Gene Bank ID
Research Area
Cell Adhesion
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Cell Biology
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Immunology

References & Citations

1. Ebens, A. et al. (2009) Cancer Res.69(6):2358-64. Article Link
2. Crocker PR et al. (2000) Immunol. 101: 342
3. Tsubata T et al. (2008) J Immunol. 180: 907
Flow Cytometry
IHC FF
Products are for research use only. Not for use in diagnostic or therapeutic procedures.