Anti-Human CD22 – Purified

This purified antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.

This purified antibody is stable when stored at 2-8°C. Do not freeze.

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Mouse Anti-Human CD22 (Clone RFB-4) recognizes Human CD22. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. Specificity: Anti-Human CD22 recognizes the (Mr 135 kDa) cytoplasmic antigen of all B-lymphocytes. The CD22 antigen is only expressed on the cell surface of mature B-lymphocytes.

The CD22 antigen is expressed on >90% of peripheral blood B-cells.² This antigen is not present on T-cells, monocytes or macrophages. CD22 is expressed on most peripheral B-cell lymphomas and leukemias including hairy cell leukemia, but not on the immature bone marrow type of leukemia.

Cluster of differentiation 22 also known as CD22 is a B-cell-restricted transmembrane protein, which acts as a negative regulator of B-cell signalling. CD22 also has lectin-like adhesive properties.² CD22 is a good target to shorten the time required for Ab production, thereby augmenting host defense against acute infectious diseases as "universal vaccination."³

CD45RO, CD75

1. Ebens, A. et al. (2009) Cancer Res.69(6):2358-64. Article Link
2. Crocker PR et al. (2000) Immunol. 101: 342
3. Tsubata T et al. (2008) J Immunol. 180: 907