Anti-Human CD22 - Purified - Leinco Technologies

Anti-Human CD22 – Purified

Product No.: C179


Clone RFB-4 Target CD22 Formats AvailableView All Product Type Monoclonal Antibody Alternate Names BL-CAM, MGC130020, SIGLEC2 Isotype IgG1 Applications FC , IHC FF


Select Product Size 50 µg — $55.00 100 µg — $93.00 200 µg — $165.00	Qty Max: Min: 1 Step: 1 Select A Size ADD TO CART Login For mAb Advantage Pricing Contact Us for Bulk Quantities


Antibody Details Product Details Reactive Species Human Host Species Mouse Product Concentration 0.5 mg/ml Formulation This purified antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative. Storage and Handling This purified antibody is stable when stored at 2-8°C. Do not freeze. Country of Origin USA Shipping Next Day Ambient RRIDAB_2828857 Applications and Recommended Usage? Quality Tested by Leinco FC The suggested concentration for this RFB-4 antibody for staining cells in flow cytometry is ≤ 1.0 μg per 10⁶ cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application. Additional Applications Reported In Literature ? IHC (Frozen) Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. Description Description Specificity Mouse Anti-Human CD22 (Clone RFB-4) recognizes Human CD22. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. Specificity: Anti-Human CD22 recognizes the (Mr 135 kDa) cytoplasmic antigen of all B-lymphocytes. The CD22 antigen is only expressed on the cell surface of mature B-lymphocytes. Background Cluster of differentiation 22 also known as CD22 is a B-cell-restricted transmembrane protein, which acts as a negative regulator of B-cell signalling. CD22 also has lectin-like adhesive properties.² CD22 is a good target to shorten the time required for Ab production, thereby augmenting host defense against acute infectious diseases as "universal vaccination."³ Antigen Distribution The CD22 antigen is expressed on >90% of peripheral blood B-cells.² This antigen is not present on T-cells, monocytes or macrophages. CD22 is expressed on most peripheral B-cell lymphomas and leukemias including hairy cell leukemia, but not on the immature bone marrow type of leukemia. Ligand/Receptor CD45RO, CD75 PubMed CD22 NCBI Gene Bank ID 933 UniProt.org Information on Uniprot.org Research Area Cell Adhesion . Cell Biology . Immunology References & Citations 1. Ebens, A. et al. (2009) Cancer Res.69(6):2358-64. Article Link 2. Crocker PR et al. (2000) Immunol. 101: 342 3. Tsubata T et al. (2008) J Immunol. 180: 907 View product citations for antibody C179 on CiteAb Technical Protocols IHC FF Certificate of Analysis Request COA

Antibody Details

Product Details

Reactive Species

Human

Host Species

Mouse

Product Concentration

0.5 mg/ml

Formulation

This purified antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.

Storage and Handling

This purified antibody is stable when stored at 2-8°C. Do not freeze.

Country of Origin

USA

Shipping

Next Day Ambient

RRIDAB_2828857

Applications and Recommended Usage?
Quality Tested by Leinco

FC The suggested concentration for this RFB-4 antibody for staining cells in flow cytometry is ≤ 1.0 μg per 10⁶ cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application.

Additional Applications Reported In Literature ?

IHC (Frozen)

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Mouse Anti-Human CD22 (Clone RFB-4) recognizes Human CD22. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. Specificity: Anti-Human CD22 recognizes the (Mr 135 kDa) cytoplasmic antigen of all B-lymphocytes. The CD22 antigen is only expressed on the cell surface of mature B-lymphocytes.

Background

Cluster of differentiation 22 also known as CD22 is a B-cell-restricted transmembrane protein, which acts as a negative regulator of B-cell signalling. CD22 also has lectin-like adhesive properties.² CD22 is a good target to shorten the time required for Ab production, thereby augmenting host defense against acute infectious diseases as "universal vaccination."³

Antigen Distribution

The CD22 antigen is expressed on >90% of peripheral blood B-cells.² This antigen is not present on T-cells, monocytes or macrophages. CD22 is expressed on most peripheral B-cell lymphomas and leukemias including hairy cell leukemia, but not on the immature bone marrow type of leukemia.

Ligand/Receptor

CD45RO, CD75

PubMed

CD22

NCBI Gene Bank ID

933

UniProt.org

Information on Uniprot.org

Research Area

Cell Adhesion

Cell Biology

Immunology

References & Citations

1. Ebens, A. et al. (2009) Cancer Res.69(6):2358-64. Article Link
2. Crocker PR et al. (2000) Immunol. 101: 342
3. Tsubata T et al. (2008) J Immunol. 180: 907

View product citations for antibody C179 on CiteAb

IHC FF

Certificate of Analysis

Request COA

Prod No.	Description
C2364	Anti-Mouse CD22 – DyLight® 488
C2365	Anti-Mouse CD22 – DyLight® 550
C2366	Anti-Mouse CD22 – DyLight® 594
C2367	Anti-Mouse CD22 – DyLight® 650
C2361	Anti-Mouse CD22 – APC
C2362	Anti-Mouse CD22 – PE
C2148	Anti-Mouse CD22 – Purified
C2359	Anti-Mouse CD22 – FITC
C2360	Anti-Mouse CD22 – Biotin

Formats Available

Prod No.	Description
C1960	Anti-Human CD22 – PerCP
C179	Anti-Human CD22 – Purified
C180	Anti-Human CD22 – Biotin
C181	Anti-Human CD22 – FITC
C1952	Anti-Human CD22 – DyLight® 488
C1953	Anti-Human CD22 – DyLight® 550
C1954	Anti-Human CD22 – DyLight® 594
C1956	Anti-Human CD22 – DyLight® 650
C1958	Anti-Human CD22 – DyLight® 755
C182	Anti-Human CD22 – PE
C318	Anti-Human CD22 – APC

Products are for research use only. Not for use in diagnostic or therapeutic procedures.

NEW Products!

Anti-Human CD22 – Purified