Anti-Human CD22 – DyLight® 550

Anti-Human CD22 – DyLight® 550

Product No.: C1953

[product_table name="All Top" skus="C1953"]

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Clone
RFB-4
Target
CD22
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
BL-CAM, MGC130020, SIGLEC2
Isotype
IgG1
Applications
FC

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Product Size
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Antibody Details

Product Details

Reactive Species
Human
Host Species
Mouse
Immunogen
Purified Recombinant Human CD22 (>98%)
Product Concentration
0.2 mg/ml
Formulation
This DyLight® 550 conjugate is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Handling
This DyLight® 550 conjugate is stable when stored at 2-8°C. Do not freeze.
Country of Origin
USA
Shipping
Next Day 2-8°C
Excitation Laser
Yellow Laser (563 nm)
Applications and Recommended Usage?
Quality Tested by Leinco
FC The suggested concentration for this RFB-4 antibody for staining cells in flow cytometry is ≤ 1.0 μg per 106 cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Mouse Anti-Human CD22 (Clone RFB-4) recognizes an epitope on Human CD22. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Antigen Distribution
The CD22 antigen is expressed on >90% of peripheral blood B-cells.1 This antigen is not present on T-cells, monocytes or macrophages. CD22 is expressed on most peripheral B-cell lymphomas and leukemias including hairy cell leukemia, but not on the immature bone marrow type of leukemia.
Background
Cluster of differentiation 22 also known as CD22 is a B-cell-restricted transmembrane protein, which acts as a negative regulator of B-cell signalling. CD22 also has lectin-like adhesive properties.1 CD22 is a good target to shorten the time required for Ab production, thereby augmenting host defense against acute infectious diseases as "universal vaccination."2

Antigen Details

Ligand/Receptor
CD45RO, CD75
PubMed
NCBI Gene Bank ID
Research Area
Cell Adhesion
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Cell Biology
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Immunology

References & Citations

1. Crocker PR et al. (2000) Immunol. 101: 342 2. Tsubata T et al. (2008) J Immunol. 180: 907
Flow Cytometry
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Formats Available

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Products are for research use only. Not for use in diagnostic or therapeutic procedures.