Clone CD28.2 has been validated for use on CODEX® using human tonsil Fresh Frozen (FF) tissue.
FF (Fresh Frozen)
Antibody and Reporter Details
≥ 2.0 mg/ml
This purified antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4.
Storage and Handling
This antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Applications and Recommended Usage?
Quality Tested by Leinco
CODEX® This CD28 (Clone CD28.2) antibody is formulated to simplify the antibody preparation needed when performing a CODEX® barcode conjugate. The suggested concentration is 0.5 mg/ml.
Country of Origin
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Clone CD28.2 recognizes an epitope on human CD28.
CD28 is expressed on thymocytes, CD4+, CD8+ peripheral T cells, NK cells.
CD28 is a 44 kD glycoprotein and a member of the Ig superfamily. In its capacity as a costimulatory receptor, CD28 produces co-stimulatory signals necessary for T cell activation and survival. CD28 is the only B7 receptor constitutively expressed on naive T cells. Without having this CD28/B7 interaction, T cells will fail to respond to their specific antigen when coming in contact with an MHC/antigen complex and thus, are said to be anergic.
NCBI Gene Bank ID
References & Citations
1. Stern, LJ. et al. (2005) Proc Natl Acad Sci U S A.102(10):3744-9 PubMed
Products are for research use only. Not for use in diagnostic or therapeutic procedures.