Anti-Human CD307e (FcRL5) [Clone 509F6] — Purified in vivo GOLD™ Functional Grade

Cells transfected with Human FcRL5.

This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.

Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.

Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles.

Next Day 2-8°C

B IP

Clone 50946 recognizes human FcRL5 within the first 3 Ig domains. Clone 50946 does not cross-react with FcRL4.

FcRL5 antibody, 509F6, recognizes Fc receptor-like 5 (FcRL5), also known as immunoglobulin superfamily receptor translocation associated 2 (IRTA2), Fc receptor homolog 5 (FcRH5), and BXMAS1. FcRL5 is a 106 kDa type I transmembrane glycoprotein containing 9 Ig-like extracellular domains, a cytoplasmic ITAM-like consensus sequence, and two cytoplasmic ITIM-like sequences¹. FcRL5 is found on most mature B cells, with the highest levels on naive and memory B cells and plasma cells^1,2. FcRL5 is an IgG receptor and binds to all IgG isotypes³, resulting in the inhibition of BCR signaling through the recruitment of SH2 domain-containing tyrosine phosphatase 1 (SHP-1) after tyrosine phosphorylation of its two ITIMs⁴. B cell malignancies, including multiple myeloma, chronic lymphocytic leukemia, mantle cell lymphoma, and hairy cell leukemia, often exhibit aberrant FCRL5 expression, indicating FcRL5 as a potential biomarker or therapeutic target^5-7.

FcRL5 is expressed on mature B cells and plasma cells.

Aggregated IgG

1. M. D. Cooper., et al. (2001). Proc. Natl. Acad. Sci. USA 98: 9772–9777
2. Koeppen H., et al. (2006) Int Immunol. 18:1363-1373
3. Colonna M., et al. (2012) J Immunol. 188(10):4741-5
4. M. D. Cooper., et al (2007) Proc. Natl. Acad. Sci. USA 104: 9770–9775
5. Dalla-Favera R., et al. (2001) Immunity. 14(3):277-89
6. Pastan I., et al. (2007) Leukemia. 21(1):169-74
7. Nagata S., et al. (2005) Clin Cancer Res. 11(1):87-96