Anti-Human CD307e (FcRL5) [Clone 509F6] — Purified in vivo PLATINUM™ Functional Grade

The clone 509F6 is used in in vivo mouse studies primarily as a functional grade preclinical antibody for research applications targeting human CD307e (FcRL5) in xenograft models.

Manufacturing and Preparation for In Vivo Use

The 509F6 antibody is specifically manufactured as an "In Vivo GOLD™" functional grade product designed for preclinical studies. This preparation involves several key features that make it suitable for in vivo applications:

• Animal-free production: The antibody is manufactured in an animal-free facility using in vitro cell culture techniques
• Low endotoxin levels: The preparation maintains endotoxin levels below 1.0 EU/mg as determined by the LAL method
• High purity: The antibody achieves ?95% monomer purity by analytical size exclusion chromatography and >95% purity by SDS-PAGE
• Multi-step purification: The manufacturing process includes protein A or G purification to ensure extremely low levels of endotoxins, leachable protein A, or aggregates

Research Applications in Mouse Models

The primary application of clone 509F6 in mouse studies appears to be in xenograft models, particularly for cancer research. The search results specifically mention its use in multiple myeloma research, where chimeric antigen receptor T cells targeting FcRH5 (CD307e) have shown robust tumor-specific responses in murine xenograft models.

Functional Properties

Clone 509F6 has important functional blocking capabilities that make it valuable for in vivo studies. The antibody can block the binding of FcRL5 to all aggregated IgG isotypes, which allows researchers to study the functional role of FcRL5 signaling pathways in vivo by preventing the natural ligand-receptor interactions.

Storage and Handling

For in vivo studies, the functional grade antibody can be stored sterile at 2-8°C for up to one month as received, or for longer-term storage, it should be aseptically aliquoted without dilution and stored at -80°C while avoiding repeated freeze-thaw cycles.

The 509F6 clone's combination of high purity, low endotoxin levels, and functional blocking activity makes it particularly suitable for studying FcRL5-mediated immune responses and potential therapeutic interventions in mouse xenograft models of human diseases, especially B cell malignancies where FcRL5 expression is often aberrant.

The correct storage temperature for sterile packaged clone 509F6 (Anti-Human CD307e / FcRL5 antibody) is 2–8°C for short-term storage (at least one week). For long-term storage, the antibody should be aseptically aliquoted without diluting and stored at –80°C. Avoid repeated freeze-thaw cycles.

If the antibody is a fluorochrome-conjugated product (e.g., PE or APC conjugates), store undiluted at 2–8°C and do not freeze, as freezing can damage fluorescent conjugates.

• For unconjugated sterile antibody: 2–8°C (short-term) or –80°C (long-term).
• For PE or APC conjugates: 2–8°C only. Do not freeze.

The antibody 509F6, which targets Fc receptor-like 5 (FcRL5), is often used in conjunction with other antibodies or proteins in various immunological studies. Here are some commonly used antibodies or proteins that might be used alongside 509F6, although specific combinations may vary based on the research context:

1. Anti-CD19 Antibodies: These are used to identify B cells and can be used alongside 509F6 to study B cell subpopulations or functions, especially since FcRL5 is differentially expressed in B cell lineages.

2. Anti-CD138 Antibodies: Targeting plasma cells, these antibodies can be combined with 509F6 to investigate plasma cell biology, as FcRL5 is upregulated on plasma cells.

3. Fc?R1 (CD64) Antibodies: Given the homology between FcRL5 and Fc?R1, studies might use both antibodies to compare their functions or interactions with immunoglobulins.

4. Anti-IgG Antibodies: These can be used to precipitate or block FcRL5 interactions with IgG isotypes, complementing the blocking capabilities of 509F6.

Without specific literature references, these suggestions are based on the known functions and applications of 509F6. However, actual combinations would depend on the research question being addressed.

Clone 509F6 is a monoclonal antibody widely cited in the scientific literature for specifically recognizing and blocking human Fc receptor-like 5 (FcRL5/CD307e), a protein with regulatory roles in B cell biology and implications in B cell malignancy research.

Key findings and uses from scientific citations include:

• Specificity and Epitope Recognition: Clone 509F6 binds with high specificity to human FcRL5, targeting an epitope within the first three Ig-like domains of the extracellular region. It does not cross-react with closely related proteins like FcRL4, enhancing its utility in studies requiring high selectivity.
• Blocking Activity: 509F6 has been shown to block FcRL5's interaction with immune complexes, specifically preventing the binding of FcRL5 to all aggregated human IgG isotypes. This has made it a valuable tool for functional studies investigating the role of FcRL5 in immunoglobulin-mediated signaling.
• Flow Cytometry Applications: The antibody is widely used in flow cytometry to characterize FcRL5 expression across B cell populations, particularly in normal and malignant B cells. Studies have shown highest FcRL5 levels on naive and memory B cells and plasma cells, and its expression is upregulated in plasma cells and some leukemias/lymphomas.
• Research in B Cell Malignancies: Aberrant FcRL5 expression detected by 509F6 is associated with multiple myeloma, chronic lymphocytic leukemia, mantle cell lymphoma, and hairy cell leukemia, supporting its use as a biomarker or potential therapeutic target in these cancers.
• Preclinical and Translational Research: Clone 509F6 has been utilized to validate FcRL5 as a cell surface antigen for immunotherapeutic strategies, including the assessment of FcRL5-targeting CAR-T cell therapies in multiple myeloma and extramedullary myeloma models. Immunofluorescence with 509F6 confirmed FcRL5 expression in relevant patient-derived samples, underscoring its clinical research relevance.

Summary Table of Key Features and Findings for Clone 509F6

Collectively, clone 509F6 is essential for precise detection, quantification, and functional interrogation of FcRL5 in both basic and translational B cell studies.