Anti-Human CD40 (Clone CPTC-CD40-4) — Purified No Carrier Protein

Human CD40 peptide sequence SCSPGFGVK

This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.

Liquid

Purified antibodies are manufactured in an animal free facility using only in vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.

This antibody may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles.

Research Use Only

2 – 8° C Wet Ice

FSAI138R-7G6 activity is directed against CD40 peptide sequence SCSPGFGVK.

CPTC-CD40-4

CD40 (also known as TNFRSF5) and CD40L (TNFSF5) are a receptor ligand pair belonging to the tumor necrosis factor receptor/TNF superfamily that functions in immune regulation¹. CD40 acts as a co-stimulatory receptor that, when interacting with its ligand CD40L on activated T cells, promotes activation, survival, production of pro-inflammatory cytokines and chemokines in dendritic cells, macrophages, and B cells, and enhances tumor antigen presentation^1,2. This in turn can further activate tumor-infiltrating lymphocytes². CD40 is a target of immunotherapy to limit the activity of antigen presenting cells and T cells in cancer, autoimmune disease, and inflammatory disease^1,2.

FSAI138R-7G6 was generated in mouse for use in immuno-MRM assays using a synthetic peptide derived from human CD40 that is carbamidomethylated at the cysteine, SC[cam]SPGFGVK³. FSAI138R-7G6 detects CD40 by Western blotting in HeLa, A549, MCF7, and H226 cell lysates, with some bands running higher than expected due to glycosylation⁴. FSAI138R-7G6 does not detect recombinant CD40 by Western blotting. FSAI138R-7G6 also does not detect CD40 by reverse phase protein array.

CD40 is expressed by B cells and in primary carcinomas.

CD40L (CD154)

1 Locksley RM, Killeen N, Lenardo MJ. Cell. F104(4):487-501. 2001.
2 Croft M, Salek-Ardakani S, Ware CF. Nat Rev Drug Discov. 23(12):939-961. 2024.
3 Whiteaker JR, Lundeen RA, Zhao L, et al. Front Immunol. 12:765898. 2021.
4 https://antibodies.cancer.gov/detail/CPTC-CD40-4