Anti-Human CD47 – (Clone B6H12) – Purified In vivo PLATINUM™ Functional Grade

Pricing & Details

Product No.C8111
Clone
B6H12
Protein
CD47
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
IAP, OA3, neutrophilin, gp42, MEM-133, CDw149, ITPG
Isotype
Mouse
IgG1 κ
Applications
FA
,
FC
,
N
Prod No.
Size
Price
Avail.
Qty
Add to cart
C8111-1mg
1 mg
$198.00
In stock
Max:
Min: 1
Step: 1
C8111-5mg
5.0 mg
$355.00
In stock
Max:
Min: 1
Step: 1
C8111-25mg
25 mg
$995.00
In stock
Max:
Min: 1
Step: 1
C8111-50mg
50 mg
$1,500.00
In stock
Max:
Min: 1
Step: 1
C8111-100mg
100 mg
$2,220.00
In stock
Max:
Min: 1
Step: 1

Antibody Details

Product Details

Reactivity Species
Human
Host Species
Mouse
Immunogen
Purified Recombinant Human CD47 (>98%)
Product Concentration
1.0 - 5.0 mg/ml
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Purity
>98% by SDS-PAGE and HPLC
Formulation
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (PBS) pH 7.2 - 7.4, 150 mM NaCl with no carrier protein, potassium or preservatives added.
Storage and Handling
This Low Endotoxin Functional Formulation conjugate is stable when stored at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
USA
Applications and Recommended Usage?
Quality Tested by Leinco
FC
Other Applications Reported In Literature ?
N
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Clone B6H12 recognizes an epitope on human CD47.
Antigen Distribution
CD47 is expressed on leukocytes, platelets, erythrocytes, epithelial cells, endothelial cells, fibroblasts, and is overexpressed in ovarian cancer, as well as many different tumor cells.
Background
CD47 is a 50 kD transmembrane protein receptor to the Ig superfamily that can manifest in four isoforms, differing only in the length of their cytoplasmic tail. CD47 is involved in various cellular processes including apoptosis, proliferation, adhesion, and migration, and plays a key role in immune and angiogenic responses. CD47 was first identified as a tumor antigen on human ovarian cancer and its expression has subsequently been found on multiple human tumor types such as acute myeloid leukemia (AML), chronic myeloid leukemia, acute lymphoblastic leukemia (ALL), non-Hodgkin’s lymphoma (NHL), multiple myeloma (MM), bladder cancer, and pediatric and adult brain tumors. CD47 interacts with membrane integrins, TSP-1, and SIRPα. The CD47/SIRPα interaction results in the inhibition of phagocytosis by macrophages thus, allowing cancer cells to multiply unchecked. Anti-CD47 antibody is a potential therapeutic target in a variety of cancers and for the treatment of pulmonary fibrosis. Anti-CD47 antibody treatment not only facilitates macrophage phagocytosis of cancer, but also promotes the activation of cancer-specific lymphocytes by acting as a beacon, flagging cancer cells which now display altered proteins to which the immune system can react.

Antigen Details

Protein
Ligand/Receptor
Non-covalently associated with ß3 integrins CD51/CD61, CD41/CD61. Thrombospondin is a ligand for CD47.
PubMed
NCBI Gene Bank ID
Research Area
Immunology
Flow Cytometry

Formats Available

Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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