Anti-Human CD47 (Clone CPTC-CD47-4) — Purified No Carrier Protein

Synthetic peptide STVPTDFSSAK

This recombinant monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.

Liquid

Recombinant antibodies are manufactured in an animal free facility using only in vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.

This antibody may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles.

Research Use Only

2 – 8° C Wet Ice

EB0123A-2C3-H1/K3 activity is directed against CD47 peptide sequence STVPTDFSSAK.

CPTC-CD47-4

In healthy cells, signal molecules stimulate programmed cell removal via various proteins, phospholipids, and abnormal glycosylation¹. However, cancer cells are able to evade phagocytic elimination, the normal method of cell removal by the innate immune system¹, due to the inhibitory antiphagocytic “don’t eat me” signal generated by CD47². The CD47 signal, which is overexpressed on cancer cells³, enables immune evasion from macrophages and other phagocytes². Since CD47 overexpression has been found on all known solid tumors and leukemias, it is a universal blocking target for cancer immunotherapy¹.

EB0123A-2C3-H1/K3 was generated in rabbit for use in immuno-MRM assays using a synthetic peptide derived from human CD47, STVPTDFSSAK⁴. EB0123A-2C3-H1/K3 does not detect CD47 by immunohistochemistry, immunofluorescence, or reverse phase protein array⁵. EB0123A-2C3-H1/K3 does detect CD47 in buffy coat, HeLa, Jurkat, A549, MCF7, and H226 whole cells lysates by Western blotting but not recombinant protein.

CD47 is a cell-surface protein with ubiquitous expression that is also overexpressed on cancer cells.

THBS1, SIRPA, FAS/CD95, SIRPG, UBQLN1, UBQLN2

1 Liu J, Wang L, Zhao F, et al. PLoS One. 10(9):e0137345. 2015.
2 Advani R, Flinn I, Popplewell L, et al. N Engl J Med. 379(18):1711-1721. 2018.
3 Maute R, Xu J, Weissman IL. Immunooncol Technol. 13:100070. 2022.
4 Whiteaker JR, Lundeen RA, Zhao L, et al. Front Immunol. 12:765898. 2021.
5 https://antibodies.cancer.gov/detail/CPTC-CD47-4