Anti-Human CD56-BX023 (Clone ERIC-1); Atto 550-RX023

Pricing & Details

Product No.C500
Clone
ERIC-1
Protein
CD56
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
NCAM, Neural cell adhesion molecule, NKH1, Leu-19
Isotype
Mouse
IgG1
Applications
CODEX®
,
IHC FFPE
Prod No.
Size
Price
Avail.
Qty
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C500-25 Tests
25 tests
$400.00
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CODEX® Details

Barcode
BX023
Reporter
Atto 550-RX023
Fluorescent Dye
ATTO 550 is in the yellow fluorescent channel with an excitation max of 554 nm and an emission max of 576 nm.
Tissue Screened
MCA Sarcoma (Tumor)
Tissue Preparation
FFPE (Formalin-Fixed Paraffin-Embedded)

Antibody and Reporter Details

Reactivity Species
Human
Host Species
Mouse
Immunogen
Human Retinoblastoma tumor
Formulation
This CODEX® barcoded antibody is formulated in phosphate buffered saline (150 mM NaCl) PBS, EDTA pH 7.2 containing 0.09% sodium azide as a preservative. The CODEX® reporter is lyophilized and formulated in 1X Tris-EDTA (TE) pH 8.0 (10 mM Tris-HCl, 1 mM disodium EDTA, pH 8.0)
Product Preparation
Manufactured in an animal-free facility using only in vitro cell culture techniques, purified by affinity chromatography, and conjugated to a specific barcode under optimal conditions.
State of Matter
CODEX® barcoded antibody - Liquid; CODEX® reporter - Lyophilized
Storage and Handling
This CODEX® barcoded antibody is stable when stored at 2-8°C for up to 1 year (do not freeze). The CODEX® reporter is stable when frozen at -20°C for up to 1 year.
Applications and Recommended Usage?
Quality Tested by Leinco
CODEX®; IHC FFPE - The suggested dilution for staining tissue in immunohistochemistry - Formalin-fixed paraffin-embedded (FFPE) is 1 μl of Anti-Human CD56 (BX023) in a final volume of 200 μl of CODEX® staining buffer.
Country of Origin
USA
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Mouse Anti-Human CD56 (Clone ERIC-1) recognizes Human CD56. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. Anti-Human CD56 recognizes the (Mr 180, 145, 125 kDa) isoforms of the human neural cell adhesion molecule (NCAM).1
Antigen Distribution
The CD56 (NCAM) antigen is expressed on most neuroectodermal derived cell lines, tissues, and tumors. NCAM is also known to be expressed on some mesodermally derived tissues such as muscle1 and on natural killer (NK) lymphocytes. The binding of ERIC-1 to both normal and neoplastic tissue is lost when tissues are conventionally fixed in formalin and embedded in paraffin. The epitope was preserved when exposed to dehydrating fixatives such as cold acetone (-20EC) for 5 min. CD56 is present on 10-25% of peripheral blood lymphocytes.
Background
Antibody CD56 can be used in studies of the neural cell adhesion molecule. This antibody may also be used to study isoforms of NCAM expressed on different tumor types. Anti-CD56 (clone ERIC-1) works well in western blot for analysis of isoforms expressed. Anti-CD56 did not react with any of the leukemias or lymphomas tested.1

CODEX® reporters are made up of a fluorescent dye and a short oligonucleotide DNA barcode called a CODEX® Tag. Fluorescent reporters enable highly specific detection of corresponding barcodes, and the use of spectrally separated dyes allow for precise signal detection in up to three distinct fluorescence channels at one time.

Antigen Details

Protein
Ligand/Receptor
Heparan sulfate
PubMed
NCBI Gene Bank ID

References & Citations

1. Bourne, K. et al. (1991) J. of Neuro-Oncology 10:111
IHC

Formats Available

Products are for research use only. Not for use in diagnostic or therapeutic procedures.