ATTO 550 is in the yellow fluorescent channel with an excitation max of 554 nm and an emission max of 576 nm.
MCA Sarcoma (Tumor)
FFPE (Formalin-Fixed Paraffin-Embedded)
Antibody and Reporter Details
Human Retinoblastoma tumor
This PhenoCycler-Fusion (CODEX)® barcoded antibody is formulated in phosphate buffered saline (150 mM NaCl) PBS, EDTA pH 7.2 containing 0.09% sodium azide as a preservative. The CODEX® reporter is lyophilized and formulated in 1X Tris-EDTA (TE) pH 8.0 (10 mM Tris-HCl, 1 mM disodium EDTA, pH 8.0)
Manufactured in an animal-free facility using only in vitro cell culture techniques, purified by affinity chromatography, and conjugated to a specific barcode under optimal conditions.
State of Matter
CODEX® barcoded antibody - Liquid; CODEX® reporter - Lyophilized
Storage and Handling
This PhenoCycler-Fusion (CODEX)® barcoded antibody is stable when stored at 2-8°C for up to 1 year (do not freeze). The CODEX® reporter is stable when frozen at -20°C for up to 1 year.
Applications and Recommended Usage?
Quality Tested by Leinco
CODEX®; IHC FFPE - The suggested dilution for staining tissue in immunohistochemistry - Formalin-fixed paraffin-embedded (FFPE) is 1 μl of Anti-Human CD56 (BX023) in a final volume of 200 μl of CODEX® staining buffer.
Country of Origin
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Mouse Anti-Human CD56 (Clone ERIC-1) recognizes Human CD56. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. Anti-Human CD56 recognizes the (Mr 180, 145, 125 kDa) isoforms of the human neural cell adhesion molecule (NCAM).1
The CD56 (NCAM) antigen is expressed on most neuroectodermal derived cell lines, tissues, and tumors. NCAM is also known to be expressed on some mesodermally derived tissues such as muscle1 and on natural killer (NK) lymphocytes. The binding of ERIC-1 to both normal and neoplastic tissue is lost when tissues are conventionally fixed in formalin and embedded in paraffin. The epitope was preserved when exposed to dehydrating fixatives such as cold acetone (-20EC) for 5 min. CD56 is present on 10-25% of peripheral blood lymphocytes.
Antibody CD56 can be used in studies of the neural cell adhesion molecule. This antibody may also be used to study isoforms of NCAM expressed on different tumor types. Anti-CD56 (clone ERIC-1) works well in western blot for analysis of isoforms expressed. Anti-CD56 did not react with any of the leukemias or lymphomas tested.1
PhenoCycler-Fusion (CODEX)® reporters are made up of a fluorescent dye and a short oligonucleotide DNA barcode called a CODEX® Tag. Fluorescent reporters enable highly specific detection of corresponding barcodes, and the use of spectrally separated dyes allow for precise signal detection in up to three distinct fluorescence channels at one time.
NCBI Gene Bank ID
References & Citations
1. Bourne, K. et al. (1991) J. of Neuro-Oncology 10:111
Products are for research use only. Not for use in diagnostic or therapeutic procedures.