Anti-Human CNTF Rα-Biotin
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Antibody DetailsProduct DetailsReactive Species Human Host Species Goat Immunogen Purified Recombinant Human CNTF Rα (>98%) Formulation This biotinylated antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 50 µg of bovine serum albumin per µg of antibody with no calcium, magnesium, or preservatives present. State of Matter Lyophilized Storage and Handling The lyophilized, biotinylated antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for up to twelve months from date of receipt. The reconstituted bioin conjugate can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted conjugate, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2828557 Applications and Recommended Usage? Quality Tested by Leinco Western Blotting: To detect Human CNTF Rα this biotin conjugate can be used at a concentration of 0.1 - 0.2 µg/ml. This biotin conjugate should be used in conjunction with compatible second-step reagents such as PN:A106 and a chromogenic substrate such as PN:T343. The detection limit for Human CNTF Rα is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:C387. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Goat Anti-Human Ciliary Neurotrophic Factor Receptor Alpha (CNTF Rα) recognizes Human CNTF Rα. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research. Background Ciliary neurotrophic factor receptor alpha (CNTF Rα) is anchored to the cell membrane by a glycosyl-phosphatidylinositol linkage and is expressed exclusively in the nervous system and skeletal muscle. One possible function of this type of linkage is to allow for the regulated release of this receptor component. Potential physiological roles for the soluble CNTF receptor alpha subunit are suggested by its presence in cerebrospinal fluid and by its release from skeletal muscle in response to peripheral nerve injury.1,2,3,4 PubMed NCBI Gene Bank ID References & Citations1. Davis, S. et al. (1991)Science 253: 59 2. Davis, S. et al. (1993)Science 259: 1736 3. Davis, S. et al. (1993)Science 260: 1805 4. Ip, NY. et al. (1992)Cell 69: 1121 Technical Protocols Certificate of Analysis |
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