Anti-Human DAN-Biotin
Anti-Human DAN-Biotin
Product No.: D346
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Target DAN Product Type Polyclonal Antibody Alternate Names Differential Screening-Selected Gene Aberrative in Neuroblastoma, Neuroblastomal Candidate Region, Suppressor of Tumorigenicity, NO3 Applications ELISA Det , WB |
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Antibody DetailsProduct DetailsReactive Species Human Host Species Goat Immunogen Purified Recombinant Human DAN (>98%) Formulation This biotinylated antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 50 µg of bovine serum albumin per µg of antibody with no calcium, magnesium, or preservatives present. State of Matter Lyophilized Storage and Handling The lyophilized, biotinylated antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for up to twelve months from date of receipt. The reconstituted bioin conjugate can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted conjugate, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2829926 Applications and Recommended Usage? Quality Tested by Leinco Western Blotting: To detect Human DAN this biotin conjugate can be used at a concentration of 0.1 - 0.2 µg/ml. This biotin conjugate should be used in conjunction with compatible second-step reagents such as PN:A106 and a chromogenic substrate such as PN:T343. The detection limit for Human DAN is 1 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:D341. ELISA Sandwich Assay: This antibody can be used as the detection antibody in a sandwich ELISA at a concentration of approximately 0.1-0.4 ng/ml. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Goat Anti-Human Differential Screening-Selected Gene Aberrative in Neuroblastoma (DAN) recognizes Human DAN. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research. Background DAN is a secreted glycoprotein related to Xenopus laevis cerberus. It is being referred to also as DAND1 [DAN domain family member 1]. The gene has been described also as NO3 gene, which shows tumor suppressor activity in cells transformed by the src oncogene.1 DAN belongs to a gene family, termed CAN family, that includes the head-inducing factor Gremlin, Cerberus, PRDC.2 DAN and several other members of the can family act as antagonists of BMP and appear to block BMP signaling by preventing bone morphogenetic proteins from interacting with their receptors. DAN has recently been demonstrated to have a tumor-suppressive activity in vitro and may have an important role in regulation of the entry of cells into the S phase.3 PubMed NCBI Gene Bank ID UniProt.org References & Citations1. Ozaki and Sakiyama (1993) Proc Natl Acad Sci U S A. 90(7):2593-7. 2. Pearce et al. (1999) Developmental Biology 209:98. 3. Sakiyama, S. et al. (1995) Biochem Biophys Res Commun. 216(1):209-15. Technical ProtocolsELISA Det  Certificate of Analysis |
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
