Anti-Human DNAM-1 (Clone 102511)

Product No.: D353


Clone 102511 Target DNAM-1 Formats AvailableView All Product Type Monoclonal Antibody Alternate Names DNAX Accessory Molecule 1, CD226, DNAM1, PTA1, TLiSA1 Isotype IgG1 Applications CyTOF® , FC , N , WB


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Antibody Details Product Details Reactive Species Human Host Species Mouse Immunogen NS0-Derived Recombinant Human DNAM-1 Extracellular Domain (Accession # Q15762) Endotoxin Level <0.1 EU/µg as determined by the LAL method Formulation This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present. Storage and Handling The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2829933 Applications and Recommended Usage? Quality Tested by Leinco Western Blotting: To detect Human DNAM-1 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Human DNAM-1 is 50 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. Flow Cytometry: Suitable for use at concentration of 0.25 µg/10⁶ cells. Additional Applications Reported In Literature ? Neutralization: This antibody is useful for neutralization of Human DNAM-1 bioactivity. The antibody dose required to neutralize 50% (ND₅₀) of the biological activity of Human DNAM-1 is 1-4 µg/ml. CyTOF-ready: Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. Description Description Specificity Mouse Anti-Human DNAX Accessory Molecule 1 (DNAM-1) (Clone 102511) recognizes an epitope on Human DNAM-1. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. Background DNAX accessory molecule 1 (DNAM-1), is a tyrosine-phosphorylated signal-transducing molecule belonging to the immunoglobulin superfamily and participates in primary adhesion during cytotoxic T lymphocyte-mediated cytotoxicity. <sup<1 DNAM-1 is a functional glycoprotein expressed on immune cells, such as natural killer and T-cells. It is activated upon interaction with its biological ligands, the poliovirus receptor and nectin-2. Signaling cascades involving stimulation of DNAM-1 lead to various biological responses, including target cell lysis and immune cell activation. DNAM-1 appears to be an integral molecule in immune responses in cancer, allergic inflammatory disorders and autoimmune diseases.² PubMed DNAM-1 NCBI Gene Bank ID 10666 UniProt.org Information on Uniprot.org References & Citations 1. Phillips, JH. et al.(1996) Immunity. 4(6):573-81. 2. Levi-Schaffer, F. et al. (2008) Curr Opin Investig Drugs. 9(5):491-6. View product citations for antibody D353 on CiteAb Technical Protocols CyTOF® N Certificate of Analysis Request COA

Antibody Details

Product Details

Reactive Species

Human

Host Species

Mouse

Immunogen

NS0-Derived Recombinant Human DNAM-1 Extracellular Domain (Accession # Q15762)

Endotoxin Level

<0.1 EU/µg as determined by the LAL method

Formulation

This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.

Storage and Handling

The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.

Country of Origin

USA

Shipping

Next Day Ambient

RRIDAB_2829933

Applications and Recommended Usage?
Quality Tested by Leinco

Western Blotting: To detect Human DNAM-1 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Human DNAM-1 is 50 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
Flow Cytometry: Suitable for use at concentration of 0.25 µg/10⁶ cells.

Additional Applications Reported In Literature ?

Neutralization: This antibody is useful for neutralization of Human DNAM-1 bioactivity. The antibody dose required to neutralize 50% (ND₅₀) of the biological activity of Human DNAM-1 is 1-4 µg/ml.
CyTOF-ready: Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Mouse Anti-Human DNAX Accessory Molecule 1 (DNAM-1) (Clone 102511) recognizes an epitope on Human DNAM-1. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Background

DNAX accessory molecule 1 (DNAM-1), is a tyrosine-phosphorylated signal-transducing molecule belonging to the immunoglobulin superfamily and participates in primary adhesion during cytotoxic T lymphocyte-mediated cytotoxicity. <sup<1
DNAM-1 is a functional glycoprotein expressed on immune cells, such as natural killer and T-cells. It is activated upon interaction with its biological ligands, the poliovirus receptor and nectin-2. Signaling cascades involving stimulation of DNAM-1 lead to various biological responses, including target cell lysis and immune cell activation. DNAM-1 appears to be an integral molecule in immune responses in cancer, allergic inflammatory disorders and autoimmune diseases.²

PubMed

DNAM-1

NCBI Gene Bank ID

10666

UniProt.org

Information on Uniprot.org