Anti-Human DNase II (CT)

PN:D273

This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.

This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.

Next Day Ambient

Rabbit Anti-Human DNase II recognizes an epitope near the C-terminus of Human DNase II. This polyclonal antibody was purified using affinity chromatography.

Apoptosis is characterized by several morphological nuclear changes including chromatin condensation and nuclear fragmentation. These changes are triggered by the activation of members of caspase family, caspase activated DNase, and several novel proteins including AIF and Acinus . DNase II causes both chromatin condensation and DNA fragmentation . The genes encoding human,^2,4 porcine,⁴ and murine⁵ DNase II have been cloned. The DNase II gene encodes a 40 kDa proenzyme. The mature enzyme consists of two non-identical subunits, the 32 kDa (a) and 12 kDa (b) chains, generated by proteolytic processing. Overexpression of DNase II induces chromatin condensation.³ DNase II is ubiquitously expressed in human tissues.

1. Zamzami, N. and Kroemer, G. (1999) Nature 401:127-8
2. Yasuda, T. et al. (1998) J Biol Chem 273:2610-6
3. Krieser, RJ. and Eastman, A. (1998) J Biol Chem 273:30909-14
4. Shiokawa, D. and Tanuma, S. (1998) Biochem Biophys Res Commun 247:864-9