Anti-Human Ephrin-A3 (Clone 80814)

Anti-Human Ephrin-A3 (Clone 80814)

Product No.: E247

[product_table name="All Top" skus="E247"]

- -
- -
Clone
80814
Target
Ephrin-A3
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
Ehk1-L, EFL-2, LERK-3, EFNA3, EPLG3
Isotype
IgG2a
Applications
WB

- -
- -
Select Product Size
- -
- -

Antibody Details

Product Details

Reactive Species
Human
Host Species
Mouse
Immunogen
Purified Recombinant Human Ephrin-A3 (Accession # AAA52368)
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Formulation
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Storage and Handling
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
Western Blotting: To detect Human Ephrin-A3 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Human Ephrin-A3 is 2 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:E198.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Mouse Anti-Human Ephrin-A3 (Clone 80814.11) recognizes an epitope on Human Ephrin-A3. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. This antibody shows approximately 5% cross-reactivity with rhEphrin-A5 and no cross-reactivity with rhEphrin-A4, rmEphrin-A1, rmEphrin-B1, rhEphrin-B2 and rhEphrin-B3.
Background
Ephrin-A3, also known as EFNA3 encodes a member of the ephrin (EPH) family. The ephrins and EPH-related receptors comprise the largest subfamily of receptor protein-tyrosine kinases and have been implicated in mediating developmental events, especially in the nervous system and in erythropoiesis. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. Ephrin-A3 encodes an EFNA class ephrin.

Antigen Details

PubMed
General Western Blot Protocol

Certificate of Analysis

- -
- -
Prod No.
Description
E247

Formats Available

- -
- -
Prod No.
Description
E247
Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.