E. coli-derived human FGF-3
<0.1 EU/µg as determined by the LAL method
This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.
Storage and Handling
The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Country of Origin
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
Western Blot: Suitable for use at concentration of 0.1 µg/mL.
Other Applications Reported In Literature ?
IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 3-10 µg/ml. The recommended secondary antibody for IHC is PN:G505. For chromogenic detection with high signal and low background use PN:D100 or PN:K107.
Neutralization: This antibody is useful for neutralization of Human FGF-3 bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Human FGF-3 (at 0.3 ng/ml) is 1-4 µg/ml.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Goat Anti-Human Fibroblast Growth Factor 3 (FGF-3) recognizes Human FGF-3. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
NCBI Gene Bank ID
References & Citations
1.) Weißenbruch, Kai et al. (2021) eLife 10(e71888):10.7554/eLife.71888 Journal Link
Products are for research use only. Not for use in diagnostic or therapeutic procedures.