Anti-Human FGF R3 (Clone 136318)

Anti-Human FGF R3 (Clone 136318)

Product No.: F1084

[product_table name="All Top" skus="F1084"]

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Clone
136318
Target
FGF R3
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
Fibroblast Growth Factor Receptor 3, ACH, Cluster of Differentiation 33 (CD333), CEK2, HSFGFR3EX, JTK4
Isotype
IgG1
Applications
N
,
WB

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Antibody Details

Product Details

Reactive Species
Human
Host Species
Mouse
Immunogen
Sf 21-Derived and NS0-Derived Recombinant Human FGF R3 Extracellular Domain
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Formulation
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Storage and Handling
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
Western Blotting: To detect Human FGF R3 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for rhFGF R3 is approximately 5 ng/lane and 50 ng/lane under non-reducing and reducing conditions, respectively. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
Additional Applications Reported In Literature ?
Neutralization: Measured by its ability to neutralize FGF R3-mediated inhibition of proliferation in the NR6R‑3T3 mouse fibroblast cell line. Raines, E.W. et al. (1985) Methods Enzymol. 109: 749.The Neutralization Dose (ND50) is typically 2.5-15 ng/mL in the presence of 6 ng/mL Recombinant Human FGF R3 (IIIc) Fc Chimera, 0.3 ng/mL Recombinant Human FGF acidic, and 10 µg/mL heparin.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Mouse Anti-Human Fibroblast Growth Factor Receptor 3 (FGF R3) (Clone 136318) recognizes an epitope on Human FGF R3. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Background
Fibroblast growth factor receptor 3 is receptor tyrosine kinases expressed on most types of cells.1 FGFR3 protein plays a role in the development and maintenance of bone and brain tissue and has several important cellular functions such as regulation of cell growth and division, determination of cell type, formation of blood vessels, wound healing, and embryo development.2 FGFR3 result in autosomal dominant human skeletal dysplasias and is a major cause of benign epidermal tumors in humans.3

Antigen Details

PubMed
NCBI Gene Bank ID

References & Citations

1. Hicks, D. et al. (1999) J Mol Neurosci. 13: 187
2. Hartmann, A. et al. (2006) J Clin Invest. 116(8):2201-2207.
3. Radvanyi, F. et al. (2005) Human Molecular Genetics 14: 1153
N
General Western Blot Protocol

Certificate of Analysis

Formats Available

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Prod No.
Description
F1084
Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.