Anti-Human FGF R3 (Clone 136318)
Pricing & Details
Antibody DetailsProduct DetailsReactivity Species Human Host Species Mouse Immunogen Sf 21-Derived and NS0-Derived Recombinant Human FGF R3 Extracellular Domain Endotoxin Level <0.1 EU/µg as determined by the LAL method Formulation This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present. Storage and Handling The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2830130 Applications and Recommended Usage? Quality Tested by Leinco Western Blotting: To detect Human FGF R3 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for rhFGF R3 is approximately 5 ng/lane and 50 ng/lane under non-reducing and reducing conditions, respectively. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. Other Applications Reported In Literature ? Neutralization: Measured by its ability to neutralize FGF R3-mediated inhibition of proliferation in the NR6R‑3T3 mouse fibroblast cell line. Raines, E.W. et al. (1985) Methods Enzymol. 109: 749.The Neutralization Dose (ND50) is typically 2.5-15 ng/mL in the presence of 6 ng/mL Recombinant Human FGF R3 (IIIc) Fc Chimera, 0.3 ng/mL Recombinant Human FGF acidic, and 10 µg/mL heparin. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Mouse Anti-Human Fibroblast Growth Factor Receptor 3 (FGF R3) (Clone 136318) recognizes an epitope on Human FGF R3. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. Background Fibroblast growth factor receptor 3 is receptor tyrosine kinases expressed on most types of cells.1 FGFR3 protein plays a role in the development and maintenance of bone and brain tissue and has several important cellular functions such as regulation of cell growth and division, determination of cell type, formation of blood vessels, wound healing, and embryo development.2 FGFR3 result in autosomal dominant human skeletal dysplasias and is a major cause of benign epidermal tumors in humans.3 Antigen DetailsProtein PubMed NCBI Gene Bank ID UniProt.org References & Citations1. Hicks, D. et al. (1999) J Mol Neurosci. 13: 187 2. Hartmann, A. et al. (2006) J Clin Invest. 116(8):2201-2207. 3. Radvanyi, F. et al. (2005) Human Molecular Genetics 14: 1153 Technical Protocols |
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