Anti-Human FGF R3 (Clone 136312)
Anti-Human FGF R3 (Clone 136312)
Product No.: F1085
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Clone 136312 Target FGF R3 Formats AvailableView All Product Type Monoclonal Antibody Alternate Names CD333, CEK, FGF R3, FGFR3, fibroblast growth factor receptor 3, HSFGFR3EX, JTK4 Isotype IgG1 Applications ICC , IHC FFPE , WB |
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Antibody DetailsProduct DetailsReactive Species Human Host Species Mouse Immunogen Sf 21-Derived and NS0-Derived Extracellular Domains of Recombinant Human FGF R3 Isoforms Formulation This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present. Storage and Handling The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2830131 Applications and Recommended Usage? Quality Tested by Leinco Western Blotting: To detect Human FGF R3 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Human FGF R3 is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. Additional Applications Reported In Literature ? IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 25 µg/ml. The recommended secondary antibody for IHC is PN:M114. For chromogenic detection with high signal and low background use PN:D100 or PN:K107. Immunocytochemistry: Suitable for use at concentration of 8-25 µg/mL. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Mouse Anti-Human Fibroblast Growth Factor Receptor 3 (FGF R3) (Clone 136312) recognizes an epitope on Human FGF R3. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. Background Fibroblast growth factor receptor 3 is receptor tyrosine kinases expressed on most types of cells.1 FGFR3 protein plays a role in the development and maintenance of bone and brain tissue and has several important cellular functions such as regulation of cell growth and division, determination of cell type, formation of blood vessels, wound healing, and embryo development.2 FGFR3 result in autosomal dominant human skeletal dysplasias and is a major cause of benign epidermal tumors in humans.3 Antigen DetailsPubMed NCBI Gene Bank ID UniProt.org References & Citations1. Hicks, D. et al. (1999) J Mol Neurosci. 13: 187 2. Hartmann, A. et al. (2006) J Clin Invest. 116(8):2201-2207. 3. Radvanyi, F. et al. (2005) Human Molecular Genetics 14: 1153 Technical ProtocolsICC IHC FFPE  |
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Formats Available
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
