Anti-Human FGF R3 (Clone 136334)
Pricing & Details
Sf 21-Derived and NS0-Derived Recombinant Human FGF-R3 Extracellular Domain
<0.1 EU/µg as determined by the LAL method
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Storage and Handling
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Country of Origin
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing FGF R3. A suggested method would be to stain cells expressing FGF R3 with 0.25 µg per 1 - 2.5 x 106 cells in a total staining volume of ≤200 µl followed by PN:M1189.
Western Blotting: To detect Human FGF R3 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Human FGF R3 is 25 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
Other Applications Reported In Literature ?
CyTOF-ready: Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Mouse Anti-Human Fibroblast Growth Factor Receptor 3 (FGF R3) (Clone 136334) recognizes an epitope on Human FGF R3. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. This antibody does not cross-react with any isoforms of rmFGF R3, rmFGF R2, rhFGF R1, rhFGF R2, or rhFGF R4.
Fibroblast growth factor receptor 3 is receptor tyrosine kinases expressed on most types of cells.1 FGFR3 protein plays a role in the development and maintenance of bone and brain tissue and has several important cellular functions such as regulation of cell growth and division, determination of cell type, formation of blood vessels, wound healing, and embryo development.2 FGFR3 result in autosomal dominant human skeletal dysplasias and is a major cause of benign epidermal tumors in humans.3
NCBI Gene Bank ID
References & Citations
1. Hicks, D. et al. (1999) J Mol Neurosci. 13: 187
2. Hartmann, A. et al. (2006) J Clin Invest. 116(8):2201-2207.
3. Radvanyi, F. et al. (2005) Human Molecular Genetics 14: 1153
Products are for research use only. Not for use in diagnostic or therapeutic procedures.