Anti-Human FGF R4

Product No.: F1080

[product_table name="All Top" skus="F1080"]

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Clone
137114
Target
FGF R4
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
Fibroblast Growth Factor Receptor 4, Cluster of Differentiation (CD334), JTK2, MGC20292, TKF
Isotype
IgG2a
Applications
WB

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Antibody Details

Product Details

Reactive Species
Human
Host Species
Mouse
Immunogen
NS0-Derived Recombinant Human FGF R4 Extracellular Domain (Accession # P22455)
Formulation
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Storage and Handling
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
Western Blotting: To detect Human FGF R4 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for rhFGF R4 is approximately 1 ng/lane and 25 ng/lane under non-reducing and reducing conditions, respectively. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Mouse Anti-Human Fibroblast Growth Factor Receptor 4 (FGF R4) (Clone 137114) recognizes an epitope on Human FGF R4. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. This antibody shows no cross-reactivity with any isoform of rhFGF R1, rhFGF R2, rhFGF R3, rmFGF R2 or rmFGF R3.
Background
Fibroblast growth factor receptor 4 (FGFR4) is a member of a family of transmembrane receptors with ligand-induced tyrosine kinase activity1 that is expressed at significant levels in almost all human prostate cancers, and expression of its ligands is ubiquitous.2 FGFR4 plays essential roles in systemic lipid and glucose homeostasis.3 FGFR4 may be an important regulator of osteogenesis with involvement in preosteoblast proliferation and differentiation as well as osteoblast functioning during intramembranous ossification.4 FGFR-4 signaling and receptor turnover are important potential therapeutic targets in prostate cancer.2

Antigen Details

PubMed
NCBI Gene Bank ID

References & Citations

1. Habuchi, T. et al. (2008) Int J Cancer 123: 2574
2. Ittmann, MM. et al. (2008) Neoplasia 10: 847
3. McKeehan, WL. et al. (2007) Diabetes 56: 2501
4. Nurcombe, V. et al. (2002) Int J Dev Biol. 46: 519
General Western Blot Protocol
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Prod No.
Description
F1080

Formats Available

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Prod No.
Description
F1080
Products are for research use only. Not for use in diagnostic or therapeutic procedures.