Anti-Human Galectin-3 (Clone 194804)

Anti-Human Galectin-3 (Clone 194804)

Product No.: G716

[product_table name="All Top" skus="G716"]

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Clone
194804
Target
Galectin-3
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
IgE-binding protein, MAC2, L-29, CPB-35
Isotype
IgG2b
Applications
WB

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Antibody Details

Product Details

Reactive Species
Human
Host Species
Mouse
Immunogen
Purified Recombinant Human Galectin-3 (Accession # P17931)
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Formulation
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Storage and Handling
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
Western Blotting: To detect Human Galectin-3 this monoclonal antibody can be used at a concentration of 0.2 µg/mL. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Human Galectin-3 is 0.5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:G589.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Specificity
Mouse Anti-Human Galectin-3 (Clone 194804) recognizes an epitope on Human Galectin-3. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Antigen Details

NCBI Gene Bank ID
General Western Blot Protocol

Certificate of Analysis

Formats Available

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Prod No.
Description
G716
Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.