Anti-Human Galectin-3 - Biotin - Leinco Technologies

Anti-Human Galectin-3 – Biotin

Product No.: G591


Target Galectin-3 Product Type Polyclonal Antibody Alternate Names LGALS3, Galactose-Specific Soluble Lectin 3, Lectin Galactoside-Binding Soluble 3, IgE-Binding Protein, MAC2, L-29, CBP35 Applications ELISA Det , FC , WB


Select Product Size 50 µg — $590.00	Qty Max: Min: 1 Step: 1 $590.00 ADD TO CART Login For mAb Advantage Pricing Contact Us for Bulk Quantities


Antibody Details Product Details Reactive Species Human Host Species Goat Immunogen Purified Recombinant Human Gal-3 (>98%) Accession # Q6IBA7 Formulation This biotinylated antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.4 containing 50 µg of bovine serum albumin per µg of antibody with no calcium, magnesium, or preservatives present. State of Matter Lyophilized Storage and Handling The lyophilized, biotinylated antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for up to twelve months from date of receipt. The reconstituted bioin conjugate can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted conjugate, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2830241 Applications and Recommended Usage? Quality Tested by Leinco Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing Gal-3. A suggested method would be to stain cells expressing Gal-3 with 50 µg per 1-2.5x 10⁶ cells in a total staining volume of 200 µl followed by PN:A104. Western Blotting: To detect Human Gal-3 this biotin conjugate can be used at a concentration of 0.1 - 0.2 µg/ml. This biotin conjugate should be used in conjunction with compatible second-step reagents such as PN:A106 and a chromogenic substrate such as PN:T343. The detection limit for Human Gal-3 is 2 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:G589. ELISA Sandwich Assay: This antibody can be used as the detection antibody in a sandwich ELISA at a concentration of approximately 0.1-0.4 µg/ml when used in conjunction with PN:G716 as the capture antibody at 2 - 8 µg/ml and an optimal second step reagent such as PN:A106 for the detection of Human Gal-3. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. Description Description Specificity Goat Anti-Human Galectin-3 recognizes Human Galectin-3. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research. Background Galectin-3 is a beta-galactoside-binding protein that regulates many biologic processes, including cell adhesion, migration, cell growth, tumor progression, metastasis, and apoptosis.¹ It is secreted from activated macrophages, basophils, and mast cells.² Galectin-3 may play an important role in the acute phase of human atopic dermatitis. PubMed Galectin-3 NCBI Gene Bank ID 3958 UniProt.org More Information on Uniprot.org References & Citations 1. Eisenthal, A. et al. (2009) Pathol Res Pract. 205(8):551-8. 2. Dahlgren, C. et al. (1998) Blood 91:3430. View product citations for antibody PRODUCT_CODE on CiteAb Technical Protocols ELISA Det Certificate of Analysis Request COA

Antibody Details

Product Details

Reactive Species

Human

Host Species

Goat

Immunogen

Purified Recombinant Human Gal-3 (>98%) Accession # Q6IBA7

Formulation

This biotinylated antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.4 containing 50 µg of bovine serum albumin per µg of antibody with no calcium, magnesium, or preservatives present.

State of Matter

Lyophilized

Storage and Handling

The lyophilized, biotinylated antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for up to twelve months from date of receipt. The reconstituted bioin conjugate can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted conjugate, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.

Country of Origin

USA

Shipping

Next Day Ambient

RRIDAB_2830241

Applications and Recommended Usage?
Quality Tested by Leinco

Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing Gal-3. A suggested method would be to stain cells expressing Gal-3 with 50 µg per 1-2.5x 10⁶ cells in a total staining volume of 200 µl followed by PN:A104.
Western Blotting: To detect Human Gal-3 this biotin conjugate can be used at a concentration of 0.1 - 0.2 µg/ml. This biotin conjugate should be used in conjunction with compatible second-step reagents such as PN:A106 and a chromogenic substrate such as PN:T343. The detection limit for Human Gal-3 is 2 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:G589.
ELISA Sandwich Assay: This antibody can be used as the detection antibody in a sandwich ELISA at a concentration of approximately 0.1-0.4 µg/ml when used in conjunction with PN:G716 as the capture antibody at 2 - 8 µg/ml and an optimal second step reagent such as PN:A106 for the detection of Human Gal-3.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Goat Anti-Human Galectin-3 recognizes Human Galectin-3. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.

Background

Galectin-3 is a beta-galactoside-binding protein that regulates many biologic processes, including cell adhesion, migration, cell growth, tumor progression, metastasis, and apoptosis.¹ It is secreted from activated macrophages, basophils, and mast cells.² Galectin-3 may play an important role in the acute phase of human atopic dermatitis.

PubMed

Galectin-3

NCBI Gene Bank ID

3958

UniProt.org

More Information on Uniprot.org