Anti-Human Galectin-3 – Biotin
Pricing & Details
Purified Recombinant Human Gal-3 (>98%) Accession # Q6IBA7
This biotinylated antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 50 µg of bovine serum albumin per µg of antibody with no calcium, magnesium, or preservatives present.
Storage and Handling
The lyophilized, biotinylated antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for up to twelve months from date of receipt. The reconstituted bioin conjugate can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted conjugate, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Country of Origin
Applications and Recommended Usage?
Quality Tested by Leinco
Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing Gal-3. A suggested method would be to stain cells expressing Gal-3 with 50 µg per 1-2.5x 106 cells in a total staining volume of 200 µl followed by PN:A104.
Western Blotting: To detect Human Gal-3 this biotin conjugate can be used at a concentration of 0.1 - 0.2 µg/ml. This biotin conjugate should be used in conjunction with compatible second-step reagents such as PN:A106 and a chromogenic substrate such as PN:T343. The detection limit for Human Gal-3 is 2 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:G589.
ELISA Sandwich Assay: This antibody can be used as the detection antibody in a sandwich ELISA at a concentration of approximately 0.1-0.4 µg/ml when used in conjunction with PN:G716 as the capture antibody at 2 - 8 µg/ml and an optimal second step reagent such as PN:A106 for the detection of Human Gal-3.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Goat Anti-Human Galectin-3 recognizes Human Galectin-3. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Galectin-3 is a beta-galactoside-binding protein that regulates many biologic processes, including cell adhesion, migration, cell growth, tumor progression, metastasis, and apoptosis.1 It is secreted from activated macrophages, basophils, and mast cells.2 Galectin-3 may play an important role in the acute phase of human atopic dermatitis.
NCBI Gene Bank ID
References & Citations
1. Eisenthal, A. et al. (2009) Pathol Res Pract. 205(8):551-8.
2. Dahlgren, C. et al. (1998) Blood 91:3430.
Products are for research use only. Not for use in diagnostic or therapeutic procedures.