Anti-Human Galectin-3

Product No.: G590


Target Galectin-3 Product Type Polyclonal Antibody Alternate Names LGALS3, Galactose-Specific Soluble Lectin 3, Lectin Galactoside-Binding Soluble 3, IgE-Binding Protein, MAC2, L-29, CBP35 Applications CyTOF® , FC , IHC FFPE , WB


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Antibody Details Product Details Reactive Species Human Host Species Goat Immunogen E. coli-derived recombinant human Galectin‑3 Formulation This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present. State of Matter Lyophilized Storage and Handling The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2830240 Applications and Recommended Usage? Quality Tested by Leinco Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing Gal-3. A suggested method would be to stain cells expressing Gal-3 with 50 µg per 1 - 2.5 x 10⁶ cells in a total staining volume of 200 µl followed by PN:G641. Western Blot: Suitable for use at concentration of 0.1 µg/mL. Additional Applications Reported In Literature ? IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 2-15 µg/ml. The recommended secondary antibody for IHC is PN:G505. For chromogenic detection with high signal and low background use PN:D100 or PN:K107. CyTOF-ready: Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. Description Description Specificity Rabbit Anti-Human Galectin-3 recognizes Human Galectin-3. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research. Background Galectin-3 is a beta-galactoside-binding protein that regulates many biologic processes, including cell adhesion, migration, cell growth, tumor progression, metastasis, and apoptosis.¹ It is secreted from activated macrophages, basophils, and mast cells.² Galectin-3 may play an important role in the acute phase of human atopic dermatitis. PubMed Galectin-3 NCBI Gene Bank ID 3958 References & Citations 1. Eisenthal, A. et al. (2009) Pathol Res Pract. 205(8):551-8. 2. Dahlgren, C. et al. (1998) Blood 91:3430. View product citations for antibody G590 on CiteAb Technical Protocols CyTOF® IHC FFPE Certificate of Analysis Request COA

Antibody Details

Product Details

Reactive Species

Human

Host Species

Goat

Immunogen

E. coli-derived recombinant human Galectin‑3

Formulation

This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.

State of Matter

Lyophilized

Storage and Handling

The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.

Country of Origin

USA

Shipping

Next Day Ambient

RRIDAB_2830240

Applications and Recommended Usage?
Quality Tested by Leinco

Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing Gal-3. A suggested method would be to stain cells expressing Gal-3 with 50 µg per 1 - 2.5 x 10⁶ cells in a total staining volume of 200 µl followed by PN:G641.
Western Blot: Suitable for use at concentration of 0.1 µg/mL.

Additional Applications Reported In Literature ?

IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 2-15 µg/ml. The recommended secondary antibody for IHC is PN:G505. For chromogenic detection with high signal and low background use PN:D100 or PN:K107.
CyTOF-ready: Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Rabbit Anti-Human Galectin-3 recognizes Human Galectin-3. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.

Background

Galectin-3 is a beta-galactoside-binding protein that regulates many biologic processes, including cell adhesion, migration, cell growth, tumor progression, metastasis, and apoptosis.¹ It is secreted from activated macrophages, basophils, and mast cells.² Galectin-3 may play an important role in the acute phase of human atopic dermatitis.

PubMed

Galectin-3

NCBI Gene Bank ID

3958