Anti-Human Galectin-3

E. coli-derived recombinant human Galectin‑3

This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.

The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.

Next Day Ambient

IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 2-15 µg/ml. The recommended secondary antibody for IHC is PN:G505. For chromogenic detection with high signal and low background use PN:D100 or PN:K107.
CyTOF-ready: Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Rabbit Anti-Human Galectin-3 recognizes Human Galectin-3. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.

Galectin-3 is a beta-galactoside-binding protein that regulates many biologic processes, including cell adhesion, migration, cell growth, tumor progression, metastasis, and apoptosis.¹ It is secreted from activated macrophages, basophils, and mast cells.² Galectin-3 may play an important role in the acute phase of human atopic dermatitis.

1. Eisenthal, A. et al. (2009) Pathol Res Pract. 205(8):551-8.
2. Dahlgren, C. et al. (1998) Blood 91:3430.