Anti-Human GDNF

Product No.: G116

[product_table name="All Top" skus="G116"]

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Target
GDNF
Product Type
Polyclonal Antibody
Alternate Names
Glial-Derived Neurotrophic Factor, ATF-1, ATF2, HFB1-GDNF
Applications
IHC FF
,
N
,
WB

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Antibody Details

Product Details

Reactive Species
Human
Host Species
Goat
Immunogen
Purified Recombinant Human GDNF (>98%)
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Formulation
This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.
State of Matter
Lyophilized
Storage and Handling
The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
Western Blot: Suitable for use at concentration of 0.1 µg/mL.
Additional Applications Reported In Literature ?
Immunohistochemistry: Suitable for the use of 5-15 µg/mL.
Neutralization: Measured by its ability to neutralize GDNF-induced proliferation in the SH‑SY5Y human neuroblastoma cell line. The Neutralization Dose (ND50) is typically 0.25-1 μg/mL in the presence of 40 ng/mL Recombinant Human GDNF.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Rabbit Anti-Human Glial-Derived Neurotrophic Factor (GDNF) recognizes Human GDNF. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Background
Glial cell derived neurotrophic factor, also known as GDNF is a glycosylated, disulfide-bonded homodimer that is a distantly related member of the transforming growth factor-beta superfamily.1 GDNF potently promotes the survival of many types of neurons, most prominently, dopaminergic and motorneurons. GDNF regulates kidney development and spermatogenesis, and it affects alcohol consumption.2

Antigen Details

PubMed
NCBI Gene Bank ID

References & Citations

1. Collins, F. et al. (1993)Science 260: 1130
2. Ron, D. et al. (2008) Proc. Natl. Acad. Sci. U.S.A. 105: 8114
IHC FF
N
General Western Blot Protocol

Certificate of Analysis

Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.