Anti-Human GDNF

Product No.: G116


Target GDNF Product Type Polyclonal Antibody Alternate Names Glial-Derived Neurotrophic Factor, ATF-1, ATF2, HFB1-GDNF Applications IHC FF , N , WB


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Antibody Details Product Details Reactive Species Human Host Species Goat Immunogen Purified Recombinant Human GDNF (>98%) Endotoxin Level <0.1 EU/µg as determined by the LAL method Formulation This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present. State of Matter Lyophilized Storage and Handling The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2830214 Applications and Recommended Usage? Quality Tested by Leinco Western Blot: Suitable for use at concentration of 0.1 µg/mL. Additional Applications Reported In Literature ? Immunohistochemistry: Suitable for the use of 5-15 µg/mL. Neutralization: Measured by its ability to neutralize GDNF-induced proliferation in the SH‑SY5Y human neuroblastoma cell line. The Neutralization Dose (ND₅₀) is typically 0.25-1 μg/mL in the presence of 40 ng/mL Recombinant Human GDNF. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. Description Description Specificity Rabbit Anti-Human Glial-Derived Neurotrophic Factor (GDNF) recognizes Human GDNF. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research. Background Glial cell derived neurotrophic factor, also known as GDNF is a glycosylated, disulfide-bonded homodimer that is a distantly related member of the transforming growth factor-beta superfamily.¹ GDNF potently promotes the survival of many types of neurons, most prominently, dopaminergic and motorneurons. GDNF regulates kidney development and spermatogenesis, and it affects alcohol consumption.² PubMed GDNF NCBI Gene Bank ID 2668 UniProt.org More Information on Uniprot.org References & Citations 1. Collins, F. et al. (1993)Science 260: 1130 2. Ron, D. et al. (2008) Proc. Natl. Acad. Sci. U.S.A. 105: 8114 View product citations for antibody G116 on CiteAb Technical Protocols IHC FF N Certificate of Analysis Request COA

Antibody Details

Product Details

Reactive Species

Human

Host Species

Goat

Immunogen

Purified Recombinant Human GDNF (>98%)

Endotoxin Level

<0.1 EU/µg as determined by the LAL method

Formulation

This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.

State of Matter

Lyophilized

Storage and Handling

The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.

Country of Origin

USA

Shipping

Next Day Ambient

RRIDAB_2830214

Applications and Recommended Usage?
Quality Tested by Leinco

Western Blot: Suitable for use at concentration of 0.1 µg/mL.

Additional Applications Reported In Literature ?

Immunohistochemistry: Suitable for the use of 5-15 µg/mL.
Neutralization: Measured by its ability to neutralize GDNF-induced proliferation in the SH‑SY5Y human neuroblastoma cell line. The Neutralization Dose (ND₅₀) is typically 0.25-1 μg/mL in the presence of 40 ng/mL Recombinant Human GDNF.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Rabbit Anti-Human Glial-Derived Neurotrophic Factor (GDNF) recognizes Human GDNF. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.

Background

Glial cell derived neurotrophic factor, also known as GDNF is a glycosylated, disulfide-bonded homodimer that is a distantly related member of the transforming growth factor-beta superfamily.¹ GDNF potently promotes the survival of many types of neurons, most prominently, dopaminergic and motorneurons. GDNF regulates kidney development and spermatogenesis, and it affects alcohol consumption.²

PubMed

GDNF

NCBI Gene Bank ID

2668

UniProt.org

More Information on Uniprot.org