Anti-Human GFRα-2 – Biotin

Anti-Human GFRα-2 – Biotin

Product No.: G693

[product_table name="All Top" skus="G693"]

Formats AvailableView All
Product Type
Polyclonal Antibody
Alternate Names
Glial Cell Line-Derived Neurotropic Factor soluble Receptor Alpha 2, GDNFRB, NRTNR-Alpha, NTNRA, RETL2, TRNR2, GFR alpha-2, GDNF R alpha-2

Product Size

Antibody Details

Product Details

Reactivity Species
Host Species
NS0-Derived Recombinant Human GFRα2 Extracellular Domain (Accession # O00451)
This biotinylated antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 50 µg of bovine serum albumin per µg of antibody with no calcium, magnesium, or preservatives present.
Storage and Handling
The lyophilized, biotinylated antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for up to twelve months from date of receipt. The reconstituted bioin conjugate can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted conjugate, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Country of Origin
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
Western Blotting: To detect Human GFRα2 this biotin conjugate can be used at a concentration of 0.1 - 0.2 µg/ml. This biotin conjugate should be used in conjunction with compatible second-step reagents such as PN:A106 and a chromogenic substrate such as PN:T343. The detection limit for Human GFRα2 is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.


Goat Anti-Human GDNF Family Receptor Alpha 2 (GFRα2) recognizes Human GFRα2. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Glial cell line-derived neurotrophic factor family receptor alpha-2, also known as GFRA2, is the cognate co-receptor for the neurotrophic factor neurturin that is selectively expressed by isolectin B(4) (IB 4)-binding nociceptive sensory neurons.1 GFRA2 is required for the development of the myenteric nerve plexus and is an excellent candidate gene for Hirschsprung disease (HSCR).2 GFRalpha2 signaling is also needed for development of the parasympathetic islet innervation that is critical for vagally induced hormone secretion.3

Antigen Details

NCBI Gene Bank ID

References & Citations

1. Lewin, GR. et al. (2002) J Physiol. 545: 43
2. Mulligan, LM. et al. (2001) Human Genetics 108: 409
3. Herzig, KH. et al. (2005) Diabetes 54: 1324
General Western Blot Protocol
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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