Anti-Human GITR Ligand

Purified Recombinant Human GITRL (Accession # Q9UNG2)

This antibody has been 0.2 µm filtered and formulated in either Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives present or lyophilized from Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.

Supplied either lyophilized or liquid

Lyophilized antibodies can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. PBS or reconstituted antibodies can be stored for at least four weeks at 2-8°C and long term storage aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.

Next Day Ambient

Blockade of Receptor-ligand Interaction: In a functional ELISA, 0.02-0.06 µg/mL of this antibody will block 50% of the binding of 10 ng/mL of biotinylated Recombinant Human GITRL Ligand to immobilized Recombinant Human GITRL/TNFRSF18 Fc Chimera coated at 2 µg/mL (100 µL/well). At 1 μg/mL, this antibody will block >90% of the binding.
CyTOF-ready:
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Mouse Anti-Human Glucocortoid-Induced Tumor necrosis factor Receptor (GITRL) (Clone 109114) recognizes an epitope on Human GITRL. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.