Anti-Human GITR Ligand

Pricing & Details

Product No.G717
Clone
109114
Protein
GITRL
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
Glucocorticoid Induced Tumor Necrosis Factor Receptor, TNFSF18
Isotype
Mouse
IgG1
Applications
B
,
CyTOF®
,
FC
Prod No.
Size
Price
Avail.
Qty
Add to cart
G717-25 µg
25 µg
$180.00
In stock
Max:
Min: 1
Step: 1
G717-100 µg
100 µg
$430.00
In stock
Max:
Min: 1
Step: 1
G717-500 µg
500 µg
$935.00
In stock
Max:
Min: 1
Step: 1
Bulk quantities available. Contact us for pricing.

Antibody Details

Product Details

Reactivity Species
Human
Host Species
Mouse
Immunogen
Purified Recombinant Human GITRL (Accession # Q9UNG2)
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Formulation
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.4 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Storage and Handling
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing GITRL. A suggested method would be to stain cells expressing GITR with 25 µg per 1-2.5x 105 cells in a total staining volume of 200 µl followed by PN:M1189. Concentration: 2.5 µg/106 cells
Other Applications Reported In Literature ?
Blockade of Receptor-ligand Interaction: In a functional ELISA, 0.02-0.06 µg/mL of this antibody will block 50% of the binding of 10 ng/mL of biotinylated Recombinant Human GITRL Ligand to immobilized Recombinant Human GITRL/TNFRSF18 Fc Chimera coated at 2 µg/mL (100 µL/well). At 1 μg/mL, this antibody will block >90% of the binding.
CyTOF-ready:
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Mouse Anti-Human Glucocortoid-Induced Tumor necrosis factor Receptor (GITRL) (Clone 109114) recognizes an epitope on Human GITRL. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Antigen Details

Protein
PubMed
NCBI Gene Bank ID
Flow Cytometry

Formats Available

Prod No.
Description
G717
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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