Anti-Human GITR Ligand

Anti-Human GITR Ligand

Product No.: G717

[product_table name="All Top" skus="G717"]

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Clone
109114
Target
GITRL
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
Glucocorticoid Induced Tumor Necrosis Factor Receptor, TNFSF18
Isotype
IgG1
Applications
B
,
CyTOF®
,
FC

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Product Size
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Antibody Details

Product Details

Reactive Species
Human
Host Species
Mouse
Immunogen
Purified Recombinant Human GITRL (Accession # Q9UNG2)
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Formulation
This antibody has been 0.2 µm filtered and formulated in either Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives present or lyophilized from Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.
State of Matter
Supplied either lyophilized or liquid
Storage and Handling
Lyophilized antibodies can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. PBS or reconstituted antibodies can be stored for at least four weeks at 2-8°C and long term storage aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing GITRL. A suggested method would be to stain cells expressing GITR with 25 µg per 1-2.5x 105 cells in a total staining volume of 200 µl followed by PN:M1189. Concentration: 2.5 µg/106 cells
Additional Applications Reported In Literature ?
Blockade of Receptor-ligand Interaction: In a functional ELISA, 0.02-0.06 µg/mL of this antibody will block 50% of the binding of 10 ng/mL of biotinylated Recombinant Human GITRL Ligand to immobilized Recombinant Human GITRL/TNFRSF18 Fc Chimera coated at 2 µg/mL (100 µL/well). At 1 μg/mL, this antibody will block >90% of the binding.
CyTOF-ready:
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Mouse Anti-Human Glucocortoid-Induced Tumor necrosis factor Receptor (GITRL) (Clone 109114) recognizes an epitope on Human GITRL. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Antigen Details

PubMed
NCBI Gene Bank ID
B
CyTOF®
Flow Cytometry
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Formats Available

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Prod No.
Description
G717
Products are for research use only. Not for use in diagnostic or therapeutic procedures.