Anti-Mouse GITR
Anti-Mouse GITR
Product No.: G673
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Clone 108619 Target GITR Formats AvailableView All Product Type Monoclonal Antibody Alternate Names TNFRSF18, AITR, GITR-D, Glucocorticoid Induced Tumor Necrosis Factor Receptor Isotype IgG2a Applications CyTOF® , FC , WB |
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Antibody DetailsProduct DetailsReactive Species Mouse Host Species Rat Immunogen NS0-Derived Recombinant Mouse GITR Extracellular Domain Formulation This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present. Storage and Handling The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day 2-8°C RRIDAB_2830262 Applications and Recommended Usage? Quality Tested by Leinco Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing GITR. A suggested method would be to stain cells expressing GITR with 0.25 µg per 1 - 2.5 x 106 cells in a total staining volume of ≤200 µl followed by PN:R485. Western Blotting: To detect Mouse GITR this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:R377 and a chromogenic substrate such as PN:T343. The detection limit for Mouse GITR is 25 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. Additional Applications Reported In Literature ? CyTOF-ready: Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Rat Anti-Mouse Glucocortocoid-Induced TNF Receptor (GITR) (Clone 108619) recognizes an epitope on Mouse GITR. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. Background GITR (glucocorticoid-induced tumor necrosis factor receptor) also known as TNFRSF18, is a surface receptor molecule that has been shown to be involved in inhibiting the suppressive activity of T-regulatory cells and extending the survival of T-effector cells.1 GITR is involved in the regulation of T-cell receptor mediated cell death. Constitutive expression of a transfected GITR gene induces resistance to apoptosis induced by anti CD3 monoclonal antibodies. GITR ligand interferes with the suppressor activity of CD4(+) CD25(+) regulatory T-cells.2 Antigen DetailsPubMed References & Citations1. Shimizu, J. et al. (2002) Nature Immunology 3:135–142. 2. Ji, Hb. et al. (2004)Journal of Immunology 172: 5823 Technical ProtocolsCyTOF®   |
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Formats Available
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
