Anti-Human HB-EGF (Clone 125923)

Product No.: H1483


Clone 125923 Target HB-EGF Formats AvailableView All Product Type Monoclonal Antibody Alternate Names Heparin-Binding EGF-Like Growth Factor, DTR, DTS, DTSF, HEGFL Isotype IgG2a Applications WB


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Antibody Details Product Details Reactive Species Human Host Species Mouse Immunogen Purified Recombinant Human HB-EGF (Accession # Q53H93) Endotoxin Level <0.1 EU/µg as determined by the LAL method Formulation This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present. Storage and Handling The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2830319 Applications and Recommended Usage? Quality Tested by Leinco Western Blotting: To detect Human HB-EGF this monoclonal antibody can be used at a concentration of 1 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Human HB-EGF is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:H1419. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. Description Description Specificity Mouse Anti-Human Heparin-Binding EGF-Like Growth Factor (HB-EGF) (Clone 125923) recognizes an epitope on Human HB-EGF. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. This antibody shows no cross-reactivity with rhAR, rhBTC, rhHRG-α, rhHRG-β, rhEGF and rhTGF-α. Background Heparin-binding EGF-like growth factor (HB-EGF) is a monomeric heparin binding O-glycosylated protein¹ and a member of the EGF family of proteins. It has been shown to play a role in wound healing, cardiac hypertrophy and heart development and function.² HB-EGF contributes to cell adhesion, invasion, and angiogenesis, which are integral to transcoelomic metastasis in ovarian cancer.³ PubMed HB-EGF References & Citations 1. Higashiyama, S. et al. (1993) Journal of Cell Biology 122: 933 2. Higashiyama, S. et al. (2004) Cytokine Growth Factor Rev. 15: 13 3. Miyamoto, S. et al. (2008) Mol Cancer Ther. 7: 3441 View product citations for antibody H1483 on CiteAb Technical Protocols Certificate of Analysis Request COA

Antibody Details

Product Details

Reactive Species

Human

Host Species

Mouse

Immunogen

Purified Recombinant Human HB-EGF (Accession # Q53H93)

Endotoxin Level

<0.1 EU/µg as determined by the LAL method

Formulation

This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.

Storage and Handling

The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.

Country of Origin

USA

Shipping

Next Day Ambient

RRIDAB_2830319

Applications and Recommended Usage?
Quality Tested by Leinco

Western Blotting: To detect Human HB-EGF this monoclonal antibody can be used at a concentration of 1 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Human HB-EGF is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:H1419.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Mouse Anti-Human Heparin-Binding EGF-Like Growth Factor (HB-EGF) (Clone 125923) recognizes an epitope on Human HB-EGF. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. This antibody shows no cross-reactivity with rhAR, rhBTC, rhHRG-α, rhHRG-β, rhEGF and rhTGF-α.

Background

Heparin-binding EGF-like growth factor (HB-EGF) is a monomeric heparin binding O-glycosylated protein¹ and a member of the EGF family of proteins. It has been shown to play a role in wound healing, cardiac hypertrophy and heart development and function.² HB-EGF contributes to cell adhesion, invasion, and angiogenesis, which are integral to transcoelomic metastasis in ovarian cancer.³

PubMed

HB-EGF

References & Citations

1. Higashiyama, S. et al. (1993) Journal of Cell Biology 122: 933
2. Higashiyama, S. et al. (2004) Cytokine Growth Factor Rev. 15: 13
3. Miyamoto, S. et al. (2008) Mol Cancer Ther. 7: 3441

View product citations for antibody H1483 on CiteAb

Certificate of Analysis

Request COA

Prod No.	Description
H1483	Anti-Human HB-EGF (Clone 125923)
H1489	Anti-Human HB-EGF – Biotin

Formats Available

Prod No.	Description
H1483	Anti-Human HB-EGF (Clone 125923)

Products are for research use only. Not for use in diagnostic or therapeutic procedures.

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Anti-Human HB-EGF (Clone 125923)