Anti-Human HB-EGF
Anti-Human HB-EGF
Product No.: H1483
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Clone 125923 Target HB-EGF Formats AvailableView All Product Type Monoclonal Antibody Alternate Names Heparin-Binding EGF-Like Growth Factor, DTR, DTS, DTSF, HEGFL Isotype IgG2a Applications WB |
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Antibody DetailsProduct DetailsReactive Species Human Host Species Mouse Immunogen Purified Recombinant Human HB-EGF (Accession # Q53H93) Endotoxin Level <0.1 EU/µg as determined by the LAL method Formulation This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present. Storage and Handling The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2830319 Applications and Recommended Usage? Quality Tested by Leinco Western Blotting: To detect Human HB-EGF this monoclonal antibody can be used at a concentration of 1 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Human HB-EGF is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:H1419. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Mouse Anti-Human Heparin-Binding EGF-Like Growth Factor (HB-EGF) (Clone 125923) recognizes an epitope on Human HB-EGF. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. This antibody shows no
cross-reactivity with rhAR, rhBTC, rhHRG-α, rhHRG-β, rhEGF and rhTGF-α. Background Heparin-binding EGF-like growth factor (HB-EGF) is a monomeric heparin binding O-glycosylated protein1 and a member of the EGF family of proteins. It has been shown to play a role in wound healing, cardiac hypertrophy and heart development and function.2 HB-EGF contributes to cell adhesion, invasion, and angiogenesis, which are integral to transcoelomic metastasis in ovarian cancer.3 Antigen DetailsPubMed References & Citations1. Higashiyama, S. et al. (1993) Journal of Cell Biology 122: 933 2. Higashiyama, S. et al. (2004) Cytokine Growth Factor Rev. 15: 13 3. Miyamoto, S. et al. (2008) Mol Cancer Ther. 7: 3441 Technical Protocols |
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
