Anti-Human Hepsin (Clone 3H10.1) – Purified in vivo PLATINUM™ Functional Grade

Pricing & Details

Product No.H5505
Clone
3H10.1
Protein
Hepsin
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
HPN, TMPRSS1, 3H10.1.2
Isotype
Mouse
IgG1 κ
Applications
ELISA
,
FC
Prod No.
Size
Price
Avail.
Qty
Add to cart
H5505- 1mg
1.0 mg
$198.00
In stock
Max:
Min: 1
Step: 1
H5505- 5mg
5.0 mg
$398.00
In stock
Max:
Min: 1
Step: 1
H5505- 25mg
25 mg
$995.00
In stock
Max:
Min: 1
Step: 1
H5505- 50mg
50 mg
$1,500.00
In stock
Max:
Min: 1
Step: 1
H5505- 100mg
100 mg
$2,500.00
In stock
Max:
Min: 1
Step: 1

Antibody Details

Product Details

Reactivity Species
Human
Host Species
Mouse
Immunogen
Human hepsin protein
Endotoxin Level
<0.5 EU/mg as determined by the LAL method
Purity
≥98% monomer by analytical SEC
>95% by SDS Page
Formulation
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Product Preparation
Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Pathogen Testing
To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Leinco’s Purified Functional PLATINUM™ antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile.
Storage and Handling
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
USA
Shipping
Next Day 2-8°C
Applications and Recommended Usage?
Quality Tested by Leinco
ELISA
FC
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Clone 3H10.1.2 is able to recognize full-length native Hepsin expressed on the cell surface in addition to the recombinant soluble form. Clones 3H1.1.1 and 1F2.1.1 bind to the same epitope as clone 3H10.1.2 and inhibit it (and each other) from binding Hepsin. Clone 3H10.1.2 and clone 2D5.1.9 bind separate epitopes and do not inhibit each other from binding Hepsin.
Antigen Distribution
Hepsin is expressed on the surface of epithelial cells including the liver, kidney, prostate, and thyroid in human tissues.
Background
Hepsin is a type II transmembrane serine protease (TTSP) expressed on the surface of epithelial cells including the liver, kidney, prostate, and thyroid in human tissues. The physiological function of hepsin is unclear, although, In vitro studies have shown that hepsin activates blood clotting factors VII, XII, and IX, pro-urokinase (pro-uPA), and pro-hepatocyte growth factor (pro-HGF). The over-expression of hepsin has been implicated in several types of cancer, especially ovarian and prostate, which makes it an attractive diagnostic marker for cancers. Most notably, hepsin has been identified as one of the most highly induced genes in prostate cancer, and this over-expression is correlated with the cancer progression and metastasis. Furthermore anti-hepsin antibodies have been shown to inhibit the invasion of human prostate cancer cells.1

Antigen Details

Protein
PubMed
NCBI Gene Bank ID

References & Citations

1. Wu,Q. and Parry, G. (2007) Front Biosci 12 5052-9
2. Kirchhofer, D. et al. (2006) J Biol Chem.281(41):30439-46.
Elisa Sandwich Protocol
Flow Cytometry

Formats Available