Anti-Human HLA-DP (MHC Class II, Clone B7/21) Monomorphic – PE

Anti-Human HLA-DP (MHC Class II, Clone B7/21) Monomorphic – PE

Product No.: H252

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Clone
B7/21
Target
HLA-DP Monomorphic
Formats AvailableView All
Product Type
Recombinant Monoclonal Antibody
Alternate Names
HLA-DP Monomorphic, HLADP, HLA-DPαβ, DPA and DPB, DPα and DPβ, DPαβ
Isotype
Human IgG1
Applications
FC

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Product Size
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Antibody Details

Product Details

Reactive Species
Human
Expression Host
HEK-293 Cells
Product Concentration
0.2 mg/ml
Formulation
This R-phycoerythrin (R-PE) conjugate is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Handling
This R-PE conjugate is stable when stored at 2-8°C. Do not freeze.
Country of Origin
USA
Shipping
Next Day 2-8°C
Excitation Laser
Blue Laser (488 nm) and/or Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Applications and Recommended Usage?
Quality Tested by Leinco
FC
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Anti-Human HLA-DP (Clone B7/21) recognizes a monomorphic epitope on human HLA-DP1, -DP2, -DP3, -DP4, and -DP5. It does not cross-react with HLA-DR or HLA-DQ.
Antigen Distribution
HLA-DP is expressed on antigen-presenting cells, including macrophages, monocytes, DCs, and B cells, and activated T cells.
Background
This Recombinant HLA-DP antibody, clone B7/21, recognizes the major histocompatibility complex (MHC) class II molecule Human Leukocyte Antigen - DP isotype (HLA-DP). MHC class II is constitutively expressed on human professional antigen-presenting cells (APCs), including macrophages/monocytes, dendritic cells (DCs), and B cells, and is induced on T cells upon activation1. HLA-DP consists of two transmembrane proteins, a 35 kDa α (heavy) chain and 29 kDa β (light) chain2 encoded by the HLA-DPA1 and HLA-DPB1 genes, respectively, located in the HLA complex of chromosome 6. The N-terminal α1 and β1 domains form the antigen-binding groove, which binds 13-25 aa peptides derived from exogenous antigens3. On APCs, MHC class II plays a critical role in the adaptive immune response by presenting phagocytosed antigens to helper CD4 T cells. The T cell receptor (TCR)/CD3 complex of CD4 T cells interacts with peptide-MHC class II, which induces CD4 T cell activation leading to the coordination and regulation of other effector cells. CD4 molecules also bind to MHC class II, which helps augment TCR signaling4. It has also been demonstrated that MHC class II express on activated T cells are capable of antigen presentation5 and can transduce signals into T cells, enhancing T cell proliferation and activity6. High HLA-DP expression is associated with an increased risk of graft-versus-host disease7. Specific alleles of HLA-DP are associated with autoimmune diseases, including rheumatoid arthritis8.

Antigen Details

Research Area
Immunology
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Innate Immunity

References & Citations

1. Holling TM, et al. (2004) Hum Immunol. 65(4):282-90.
2. Mitaksov V & Fremont DH. (2006) J Biol Chem. 281(15):10618-25.
3. Wieczorek M, et al. (2017) Front Immunol. 8:292.
4. Artyomov MN, et al. (2010) Proc Natl Acad Sci USA. 107(39):16916-16921.
5. Barnaba V, et al. (1994) Eur J Immunol. 24(1):71-5.
6. Di Rosa F, et al. (1993) Hum Immunol. 38(4):251-60.
7. Petersdorf EW, et al. (2015) N Engl J Med. 373(7):599-609.
8. Raychaudhuri S, et al. (2012) Nat Genet. 44(3):291-6.
Flow Cytometry
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Formats Available

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Products are for research use only. Not for use in diagnostic or therapeutic procedures.