Anti-Human HLA-DP (MHC Class II) Monomorphic – Purified

Pricing & Details

Product No.H127
Clone
B7/21
Protein
HLA-DP Monomorphic
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
HLA-DP Monomorphic, HLADP, HLA-DPαβ, DPA and DPB, DPα and DPβ, DPαβ
Isotype
Mouse
IgG3
Applications
FC
Prod No.
Size
Price
Avail.
Qty
Add to cart
H127-50 µg
50 µg
$50.00
In stock
Max:
Min: 1
Step: 1
H127-100 µg
100 µg
$85.00
In stock
Max:
Min: 1
Step: 1
H127-200 µg
200 µg
$150.00
In stock
Max:
Min: 1
Step: 1

Antibody Details

Product Details

Reactivity Species
Human
Host Species
Mouse
Product Concentration
0.5 mg/ml
Formulation
This purified antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Handling
This antibody is stable when stored at 2-8°C. Do not freeze.
Country of Origin
USA
Applications and Recommended Usage?
Quality Tested by Leinco
FC
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Clone B7/21 recognizes a monomorphic epitope on human HLA-DP1, -DP2, -DP3, -DP4, and -DP5. It does not cross-react with HLA-DR or HLA-DQ.
Antigen Distribution
HLA-DP is expressed on antigen-presenting cells, including macrophages, monocytes, DCs, and B cells, and activated T cells.
Background
HLA-DP antibody, clone B7/21, recognizes the major histocompatibility complex (MHC) class II molecule Human Leukocyte Antigen - DP isotype (HLA-DP). MHC class II is constitutively expressed on human professional antigen-presenting cells (APCs), including macrophages/monocytes, dendritic cells (DCs), and B cells, and is induced on T cells upon activation1. HLA-DP consists of two transmembrane proteins, a 35 kDa α (heavy) chain and 29 kDa β (light) chain2 encoded by the HLA-DPA1 and HLA-DPB1 genes, respectively, located in the HLA complex of chromosome 6. The N-terminal α1 and β1 domains form the antigen-binding groove, which binds 13-25 aa peptides derived from exogenous antigens3. On APCs, MHC class II plays a critical role in the adaptive immune response by presenting phagocytosed antigens to helper CD4 T cells. The T cell receptor (TCR)/CD3 complex of CD4 T cells interacts with peptide-MHC class II, which induces CD4 T cell activation leading to the coordination and regulation of other effector cells. CD4 molecules also bind to MHC class II, which helps augment TCR signaling4. It has also been demonstrated that MHC class II express on activated T cells are capable of antigen presentation5 and can transduce signals into T cells, enhancing T cell proliferation and activity6. High HLA-DP expression is associated with an increased risk of graft-versus-host disease7. Specific alleles of HLA-DP are associated with autoimmune diseases, including rheumatoid arthritis8.

Antigen Details

Research Area
Immunology
.
Innate Immunity

References & Citations

1. Holling TM, et al. (2004) Hum Immunol. 65(4):282-90.
2. Mitaksov V & Fremont DH. (2006) J Biol Chem. 281(15):10618-25.
3. Wieczorek M, et al. (2017) Front Immunol. 8:292.
4. Artyomov MN, et al. (2010) Proc Natl Acad Sci USA. 107(39):16916-16921.
5. Barnaba V, et al. (1994) Eur J Immunol. 24(1):71-5.
6. Di Rosa F, et al. (1993) Hum Immunol. 38(4):251-60.
7. Petersdorf EW, et al. (2015) N Engl J Med. 373(7):599-609.
8. Raychaudhuri S, et al. (2012) Nat Genet. 44(3):291-6.
Flow Cytometry

Formats Available

Products are for research use only. Not for use in diagnostic or therapeutic procedures.