Anti-Human HLA-DR Monomorphic – Purified (PhenoCyler-Fusion (CODEX)® Ready)

Anti-Human HLA-DR Monomorphic – Purified (PhenoCyler-Fusion (CODEX)® Ready)

Product No.: H500

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Clone
L243
Target
HLA-DR Monomorphic
Formats AvailableView All
Product Type
Monoclonal Antibody
Isotype
IgG2a
Applications
IHC FF
,
PhenoCycler®

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Product Size
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CODEX® Details

Validation Notes
Clone L243 has been validated for use on CODEX® using human tonsil Fresh Frozen (FF) tissue.
Tissue Screened
Tonsil
Tissue Preparation
FF (Fresh Frozen)

Antibody and Reporter Details

Reactivity Species
Baboon
Chimpanzee
Cynomolgus Monkey
Marmoset
Rhesus Monkey
Squirrel Monkey
Canine
Human
Host Species
Mouse
Concentration
0.5 mg/ml
Formulation
This purified antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4.
Storage and Handling
This antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Applications and Recommended Usage?
Quality Tested by Leinco
CODEX® This HLA-DR (Clone L243) antibody is formulated to simplify the antibody preparation needed when performing a CODEX® barcode conjugate. The suggested concentration is 0.5 mg/ml.
RRID
AB_2893765
Country of Origin
USA
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Anti-Human HLA-DR recognizes the HLA-DR class II major histocompatibility antigen. HLA-DR is a transmembrane glycoprotein composed of alpha and beta subunits with molecular weights of 36 kDa and 27 kDa, respectively.1 Anti HLA-DR (Clone L243) reacts with a monomorphic determinant and does not cross react with HLA-DQ or HLA-DP.2
Antigen Distribution
The HLA-DR antigen is present on B-lymphocytes, activated T-lymphocytes and activated natural killer (NK) lymphocytes. This antigen is also expressed on monocytes, macrophages, human progenitor cells and B-cell lymphomas.

Antigen Details

Ligand/Receptor
CD3/TCR, CD4

References & Citations

1. Wright, TM. et al. (2001) Arthritis Rheum. 44(7):1654-9. Article Link
2. Lampson, LA. et al. (1980) J. Immunol. 125:393
3. Yu, D. et al. (1980) J. Exp. Med. 151:91
4. Warnke, R. et al. (1980) N. Eng. J. Med. 303:293
5. Wilkinson, KA. et al. (2005) Infect Immun. 74(5):2991-8
IHC
PhenoCycler®
Products are for research use only. Not for use in diagnostic or therapeutic procedures.