Anti-Human HLA-DR (MHC Class II) (Clone L243) – Purified in vivo PLATINUM™ Functional Grade

Anti-Human HLA-DR (MHC Class II) (Clone L243) – Purified in vivo PLATINUM™ Functional Grade

Product No.: H461

[product_table name="All Top" skus="H261"]

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Clone
L243
Target
HLA-DR
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
Major Histocompatibility Class II, MHC class II, HLA-DR Monomorphic
Isotype
IgG2a
Applications
B
,
CyTOF®
,
Depletion
,
FC
,
IHC FF
,
in vivo
,
IP
,
PhenoCycler®
,
WB

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Select Product Size
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Antibody Details

Product Details

Reactive Species
Baboon
Chimpanzee
Cynomolgus Monkey
Marmoset
Rhesus Monkey
Squirrel Monkey
Canine
Human
Host Species
Mouse
Immunogen
Unknown
Product Concentration
≥ 5.0 mg/ml
Endotoxin Level
<0.5 EU/mg as determined by the LAL method
Purity
≥98% monomer by analytical SEC
>95% by SDS Page
Formulation
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Product Preparation
Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Pathogen Testing
To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Leinco’s Purified Functional PLATINUM<sup>TM</sup> antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile.
Storage and Handling
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
USA
Shipping
Next Day 2-8°C
Applications and Recommended Usage?
Quality Tested by Leinco
FC The suggested concentration for this HLA-DR (Clone L243) antibody for staining cells in flow cytometry is ≤ 0.5 μg per 106 cells in a volume of 100 μl or 100μl of whole blood. Titration of the reagent is recommended for optimal performance for each application.
WB The suggested concentration for this HLA-DR (Clone L243) antibody for use in western blotting is 1-10 μg/ml.
Additional Applications Reported In Literature ?
IHC FF
CyTOF®
B
Depletion
IP
Additional Reported Applications For Relevant Conjugates ?
PhenoCycler-Fusion (CODEX)®
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Clone L243 recognizes a conformational epitope on the human MHC class II molecule HLA-DRα, which depends on the correct folding of the αβ heterodimer1. It does not cross-react with HLA-DP or HLA-DQ.
Antigen Distribution
HLA-DR is expressed on antigen-presenting cells, including macrophages, monocytes, DCs, and B cells, and activated T cells.
Background
HLA-DR antibody, clone L243, recognizes the major histocompatibility complex (MHC) class II molecule Human Leukocyte Antigen - DR isotype (HLA-DR). MHC class II is constitutively expressed on human professional antigen-presenting cells (APCs), including macrophages/monocytes, dendritic cells (DCs), and B cells, and is induced on T cells upon activation2. HLA-DR consists of two transmembrane proteins, a 35 kDa α (heavy) chain and 29 kDa β (light) chain3 encoded by the HLA-DRA and HLA-DRB1, HLA-DRB3, HLA-DRB4, and HLA-DRB5 genes, respectively, located in the HLA complex of chromosome 6. The N-terminal α1 and β1 domains form the antigen-binding groove, which binds 13-25 aa peptides derived from exogenous antigens4. On APCs, MHC class II plays a critical role in the adaptive immune response by presenting phagocytosed antigens to helper CD4 T cells. The T cell receptor (TCR)/CD3 complex of CD4 T cells interacts with peptide-MHC class II, which induces CD4 T cell activation leading to the coordination and regulation of other effector cells. CD4 molecules also bind to MHC class II, which helps augment TCR signaling5. It has also been demonstrated that MHC class II express on activated T cells are capable of antigen presentation6 and can transduce signals into T cells, enhancing T cell proliferation and activity7. HLA-DR expression is a marker of T cell activation and correlates with disease activity in patients with autoimmune disease8 and rapid progression in HIV infection9. Specific alleles of HLA-DR are associated with autoimmune diseases, including rheumatoid arthritis10.

Antigen Details

Ligand/Receptor
CD3/TCR, CD4
Function
Peptide presentation
PubMed
NCBI Gene Bank ID
Research Area
Immunology
.
Innate Immunity

References & Citations

1. Moro M, Cecconi V, Martinoli C, et al. (2005) BMC Immunol. 6:24
2. Holling TM, et al. (2004) Hum Immunol. 65(4):282-90
3. Mitaksov V, (2006) J Biol Chem. 281(15):10618-25
4. Wieczorek M, et al. (2017) Front Immunol. 8:292
5. Artyomov MN, et al. (2010) Proc Natl Acad Sci USA. 107(39):16916-16921
6. Barnaba V, et al. (1994) Eur J Immunol. 24(1):71-5
7. Di Rosa F, et al. (1993) Hum Immunol. 38(4):251-60
8. Viallard JF, et al. (2001) Clin Exp Immunol. 125(3):485-491
9. Langford SE, Ananworanich J, Cooper DA. (2007) AIDS Res Ther. 2007;4:11
10. Gough SC, Simmonds MJ. (2007) Curr Genomics. 8(7):453-465
B
CyTOF®
Depletion
Flow Cytometry
IHC FF
in vivo Protocol
Immunoprecipitation Protocol
PhenoCycler®
General Western Blot Protocol
Products are for research use only. Not for use in diagnostic or therapeutic procedures.