Anti-Human ICAM-1 – FITC

Pricing & Details

Product No.C169
Clone
15.2
Protein
Inter-Cellular Adhesion Molecule 1
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
BB2, CD54, P3.58
Isotype
Mouse
IgG1
Applications
FC
Prod No.
Size
Price
Avail.
Qty
Add to cart
C169-50 µg
50 µg
$77.00
In stock
Max:
Min: 1
Step: 1
C169-100 µg
100 µg
$148.00
In stock
Max:
Min: 1
Step: 1
C169-200 µg
200 µg
$264.00
In stock
Max:
Min: 1
Step: 1
Bulk quantities available. Contact us for pricing.

Antibody Details

Product Details

Reactivity Species
Human
Host Species
Mouse
Immunogen
Purified Recombinant Human ICAM-1 (>98%)
Product Concentration
0.2 mg/ml
Formulation
This Fluorescein (FITC) conjugate is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Handling
This Fluorescein conjugate is stable when stored at 2-8°C. Do not freeze.
Country of Origin
USA
Shipping
Next Day Ambient
Excitation Laser
Blue Laser (490 nm)
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Mouse Anti-Human CD54 (Clone 15.2) recognizes Human CD54. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. Anti-Human CD54 (ICAM-1) recognizes the (Mr 85-115 kDa) intercellular adhesion molecule ICAM-1 which permits antigen-independent adhesion between lymphocytes and their targets. The LFA-1 binding site is located in domain 1 of ICAM-1.1,2
Antigen Distribution
ICAM-1 is a membrane glycoprotein with a wide tissue distribution that includes vascular endothelium and many cells of the immune system. CD54 is weakly expressed on resting peripheral blood lymphocytes. Upon activation by mitogens, the CD54 antigen is strongly expressed on B-cells, T-cells, macrophages and granulocytes. CD54 is the receptor for rhinoviruses and malaria.1
Background
Intercellular adhesion molecule-1 (ICAM-1) is one of an important cell adhesion molecules (CAMs) family transmembrane glycoprotein1 and is critical for the firm arrest and transmigration of leukocytes out of blood vessels and into tissues. ICAM-1 is constitutively present on endothelial cells, but its expression is increased by proinflammatory cytokines.2 ICAM-1 is a marker of endothelial dysfunction leading to damaging vascular disorders, in umbilical and placental vascular tissue of gestational pregnancies.3

Antigen Details

NCBI Gene Bank ID
Research Area
Cell Biology
.
Costimulatory Molecules
.
Immunology
.
Innate Immunity
.
Neuroscience
.
Neuroscience Cell Markers
.
Stem Cell

References & Citations

1. Li S et al. (2009) Biochem Biophys Res Commun. 381: 459 2. Wolf S et al. (2009) Pharmacol Rep 61: 22 3. Ozcan U et al. (2009) Arch Gynecol Obstet.
Flow Cytometry

Formats Available

Products are for research use only. Not for use in diagnostic or therapeutic procedures.