Anti-Human ICAM-1 – Purified in vivo PLATINUM™ Functional Grade

Pricing & Details

Product No.C672
Clone
15.2
Protein
ICAM-1
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
BB2, CD54, P3.58
Isotype
Mouse
IgG1
Applications
FC
,
IHC FFPE
,
IP
,
WB
Prod No.
Size
Price
Avail.
Qty
Add to cart
C672-1.0 mg
1.0 mg
$225.00
In stock
Max:
Min: 1
Step: 1
C672-5.0 mg
5.0 mg
$445.00
In stock
Max:
Min: 1
Step: 1
C672-25 mg
25 mg
$1,285.00
In stock
Max:
Min: 1
Step: 1
C672-50 mg
50 mg
$2,045.00
In stock
Max:
Min: 1
Step: 1
C672-100 mg
100 mg
$2,800.00
In stock
Max:
Min: 1
Step: 1
Bulk quantities available. Contact us for pricing.

Antibody Details

Product Details

Reactivity Species
Human
Host Species
Mouse
Immunogen
Human infant thymocytes and Sezary cells
Product Concentration
≥ 5.0 mg/ml
Endotoxin Level
<0.5 EU/mg as determined by the LAL method
Purity
≥98% monomer by analytical SEC
>95% by SDS Page
Formulation
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Product Preparation
Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Pathogen Testing
To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Leinco’s Purified Functional PLATINUM™ antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile.
Storage and Handling
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
USA
Shipping
Next Day 2-8°C
Applications and Recommended Usage?
Quality Tested by Leinco
FC
WB
Other Applications Reported In Literature ?
IHC FFPE
IP
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Mouse Anti-Human Inter-Cellular Adhesion Molecule 1 (Clone 15.2) recognizes an epitope on Human ICAM-1. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Antigen Distribution
CD54 is present on endothelial cells, lymphocytes, epithelial cells, dendritic cells and keratinocytes.
Background
ICAM-1 is a 55 kDa glycoprotein that is part of the Ig superfamily. It is heavily glycosylated to form 75 kDa to 115 kDa. ICAM-1 is known to be an adhesion and viral entry molecule, and its long suspected involevement in signal transduction is being elucidated. The signal-transducing functions of ICAM-1 appear to be mainly associated with proinflammatory pathways. Furthermore, ICAM-1 signaling appears to act as a beacon for inflammatory immune cells such as macrophages and granulocytes bringing about inflammation via lymphocyte trafficking. ICAM-1 is essential for the transmigration of leukocytes out of blood vessels and into tissues, and is a marker of endothelial dysfunction leading to damaging vascular disorders in umbilical and placental vascular tissue of gestational pregnancies. ICAM-1 is the receptor for rhinoviruses (the cause of most common colds) and malaria, and plays an inflammatory role in ocular allergies.

Antigen Details

Protein
PubMed
NCBI Gene Bank ID

References & Citations

1. Eaton, JW. et al. (1998) J Immunol. 161(8):4268-75. Article Link
2. Schaper, W. et al. (2000) Virchows Arch. 436(3):257-70. Article Link
3. Piela-Smith, TH. et al. (2000) J Immunol.165(9):5255-61. Article Link
4. Li, S. et al. (2009) Biochem Biophys Res Commun. 381: 459
5. Wolf, S. et al. (2009) Pharmacol Rep 61: 22
6. Ozcan, U. et al. (2010) Arch Gynecol Obstet. 281(1):71-6.
Flow Cytometry
IHC
Immunoprecipitation Protocol
General Western Blot Protocol

Formats Available