Anti-Human CD275 (ICOS Ligand) (Clone CPTC-ICOSLG-1) – Purified No Carrier Protein

ICOSLG synthetic peptide GLYDVVSVLR

This recombinant monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.

Liquid

Recombinant antibodies are manufactured in an animal free facility using only in vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.

This antibody may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles.

Research Use Only

2 – 8° C Wet Ice

EB0136C-2D3-H2/K2 activity is directed against ICOSLG peptide sequence GLYDVVSVLR.

CPTC-ICOSLG-1

ICOSLG (CD275) is the ligand for ICOS (CD278), a member of the CD28 family of T cell co- stimulatory molecules that induces activated T cells to proliferate, secrete cytokines, and express CD40 ligand as well as helps B cells to generate IgM and IgG¹. ICOSLG is a member of the B7 superfamily and when paired with ICOS is involved in T cell – APC interactions and regulates humoral immunity. Autosomal recessive defects in ICOSLG cause recurrent respiratory tract infections as part of a combined immunodeficiency syndrome, with impaired differentiation of circulating B cells and abnormal circulating Tfh-lineage cells. Additionally, ICOSLG deficiency can affect expression of E-selectin and thereby impair transendothelial migration of T lymphocytes.

EB0136C-2D3-H2/K2 was generated in rabbit for use in immuno-MRM assays using a synthetic peptide derived from residues 195-204 of human ICOSLG, GLYDVVSVLR². EB0136C-2D3- H2/K2 can also detect ICOSLG by immunohistochemistry and NCI60 protein array³. In Western blots, EB0136C-2D3-H2/K2 can detect ICOSLG as a recombinant protein and in cell lysates of buffy coat cells but does not detect ICOSLG in cell lysates of other cells. EB0136C-2D3-H2/K2 does not detect ICOSLG by immunofluorescence.

ICOSLG is extensively expressed in both lymphatic and non-lymphatic tissues. ICOSLG is expressed at low levels in naïve B cells and monocytes in peripheral blood mononuclear cells.

ICOS

1 Roussel L, Vinh DC. Curr Opin Immunol. 72:21-26. 2021.
2 Whiteaker JR, Lundeen RA, Zhao L, et al. Front Immunol. 12:765898. 2021.
3 https://antibodies.cancer.gov/detail/CPTC-ICOSLG-1