Purified Recombinant Human IL-1 RII (Accession # P27930)
<0.1 EU/µg as determined by the LAL method
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Storage and Handling
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Country of Origin
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
Western Blotting: To detect Human IL-1 RII this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Human IL-1 RII is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is I-555.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Mouse Anti-Human Interleukin-1 Soluble Receptor Type II (IL-1 sRII) (Clone 34117) recognizes an epitope on Human IL-1 sRII. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. This antibody shows no cross-reactivity with rhIL-1 RI, rhIL-18 R or rmIL-18 R.
Interleukin 1 receptor type II (IL1R2), also known as CD121b (Cluster of Differentiation 121b), is a transmembrane protein found on B lymphocytes, neutrophils, monocytes, large granular leukocytes and endothelial cells. It is a decoy receptor known for its ability to buffer IL-1 effects.1 IL-1RII is involved in the control of the proinflammatory state that takes place in the endometrium during the premenstrual and menstrual periods.2 IL-1RII can neutralize IL-1 beta and counteract its effect on endometrial stromal cells, and may provide a new clinical strategy for the treatment of endometriosis.3
References & Citations
1. Akoum, A. et al. (2005) Hum Rep. 20: 1177
2. Akoum, A. et al. (2001) Biol Rep. 65: 890
3. Liu, J. et al. (2008) Fertil Steril. 89: 42
Products are for research use only. Not for use in diagnostic or therapeutic procedures.