Anti-Human IL-1 RII – Biotin

Anti-Human IL-1 RII – Biotin

Product No.: I-765

[product_table name="All Top" skus="I-765"]

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Target
IL-1 sRII
Product Type
Polyclonal Antibody
Alternate Names
IL1R2, CD121b, IL1RB, MGC47725, Interleukin-1 Soluble Receptor Type II
Applications
ELISA Det
,
WB

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Product Size
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Antibody Details

Product Details

Reactive Species
Human
Host Species
Goat
Immunogen
Purified Recombinant Human IL-1 RII (Accession # P27930)
Formulation
This biotinylated antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 50 µg of bovine serum albumin per µg of antibody with no calcium, magnesium, or preservatives present.
State of Matter
Lyophilized
Storage and Handling
The lyophilized, biotinylated antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for up to twelve months from date of receipt. The reconstituted bioin conjugate can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted conjugate, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
Western Blotting: To detect Human IL-1 RII this biotin conjugate can be used at a concentration of 0.1 - 0.2 µg/ml. This biotin conjugate should be used in conjunction with compatible second-step reagents such as PN:A106 and a chromogenic substrate such as PN:T343. The detection limit for Human IL-1 RII is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:I-555.
ELISA Sandwich Assay: This antibody can be used as the detection antibody in a sandwich ELISA at a concentration of approximately 0.1-0.4 µg/ml when used in conjunction with PN:I-690 as the capture antibody at 2-8 µg/ml and an optimal second step reagent such as PN:A106 for the detection of Human IL-1 RII.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Goat Anti-Human Interleukin 1 Soluble Receptor Type II (IL-1 RII) recognizes Human IL-1 RII. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Background
Interleukin 1 receptor type II (IL1R2), also known as CD121b (Cluster of Differentiation 121b), is a transmembrane protein found on B lymphocytes, neutrophils, monocytes, large granular leukocytes and endothelial cells. It is a decoy receptor known for its ability to buffer IL-1 effects.1 IL-1RII is involved in the control of the proinflammatory state that takes place in the endometrium during the premenstrual and menstrual periods.2 IL-1RII can neutralize IL-1 beta and counteract its effect on endometrial stromal cells, and may provide a new clinical strategy for the treatment of endometriosis.3

Antigen Details

PubMed
NCBI Gene Bank ID

References & Citations

1. Akoum, A. et al. (2005) Hum Rep. 20: 1177
2. Akoum, A. et al. (2001) Biol Rep. 65: 890
3. Liu, J. et al. (2008) Fertil Steril. 89: 42
ELISA Det
General Western Blot Protocol
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.