Purified Recombinant Human IL-1 RII (>98%)
<0.1 EU/µg as determined by the LAL method
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Storage and Handling
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Country of Origin
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Applications and Recommended Usage?
Quality Tested by Leinco
ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 2-8 µg/ml. A suitable detection antibody is PN:I-765 at a concentration of approximately 0.1-0.4 µg/ml. A suggested standard for this assay is PN:I-555.
Western Blotting: To detect Human IL-1 RII this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Human IL-1 RII is 25 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:I-555.
Other Applications Reported In Literature ?
Neutralization: This antibody is useful for neutralization of Human IL-1 RII bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Human IL-1 RII (at 2.0 ng/ml) is 5 - 20 µg/ml.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Mouse Anti-Human Interleukin 1 Soluble Receptor Type II (IL-1 RII) (Clone 34141) recognizes an epitope on Human IL-1 RII. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Interleukin 1 receptor type II (IL1R2), also known as CD121b (Cluster of Differentiation 121b), is a transmembrane protein found on B lymphocytes, neutrophils, monocytes, large granular leukocytes and endothelial cells. It is a decoy receptor known for its ability to buffer IL-1 effects.1 IL-1RII is involved in the control of the proinflammatory state that takes place in the endometrium during the premenstrual and menstrual periods.2 IL-1RII can neutralize IL-1 beta and counteract its effect on endometrial stromal cells, and may provide a new clinical strategy for the treatment of endometriosis.3
NCBI Gene Bank ID
References & Citations
1. Akoum, A. et al. (2005) Hum Rep. 20: 1177
2. Akoum, A. et al. (2001) Biol Rep. 65: 890
3. Liu, J. et al. (2008) Fertil Steril. 89: 42
Products are for research use only. Not for use in diagnostic or therapeutic procedures.